甲状腺细胞的~3H-TdR摄取量测定的细胞因素影响

本文用TSH刺激FRTLS鼠甲状腺细胞,观察~3H-TdR掺入DNA的量,表达为每微克DNA内的~3H的每分钟放射计数(CPM/μgDNA)。细胞对刺激物的反应能力表达为刺激组细胞的~3H-TdR摄取值CPM/μgDNA除以对照组的CPM/μgDNA所得的“%的对照值”。-180℃冷冻2个月复苏的细胞对TSH200和2000μu/ml的反应能力分别为2741%和3028%,作为对比组的连续80天TSH培养的细胞反应能力分别为736%和719%,两组间存在统计学的显著差异。这提示用长期冷冻法可使因长期TSH培养而降低反应能力的细胞恢复良好的反应性。实验所需细胞数量应用一次种植、不经TSH增殖培养法,亦可提高细胞的反应性。

~3H-THYMIDINE UPTAKE ASSAY OF FRTL5 CELLSI THE INFLUENCE OF CELL FACTORS

The amount of ~3H-TdR incorporation into DИA of FRTL5 cells was expressed as CPM/μgDИA. THe responsibity of FRTL5 cells to stimulators such as TSH was expressed as % against thJ control CPM/μgDNA of the experimental group divided by that of the control. The responsibilities of FRTL5 cells which were recovered after being fr-o zen under-180℃ for a long time, to TSH 200μg/ml were 2741% and 3028% respectively, and the responsibilities of cells cultured in TSH media continuously for a long time, to TSH 200μu/ml and 2000μu/ml were 736% and 719% respectively. There was statistically significant differences between the two groups of the cells mentioned above. This showed that freezing FRTL5 Cells for a long time Such as 2 months was able to recover the decreased responsibility of the Cells due to a long continuous TSH culture.