白细胞介素-2协同白细胞介素-18活化自然杀伤细胞活性的作用

目的研究白细胞介素-2(IL-2)和白细胞介素-18(IL-18)诱导自然杀伤(NK)细胞活化和抗骨髓瘤细胞活性的协同作用。方法流式细胞仪方法检测NK细胞表面CD69的表达;阴性细胞纯化法用于获得高纯度的NK细胞;ELISA测定NK细胞培养上清液中干扰素(IFN-γ)的浓度;标准铬51释放试验评估NK细胞杀伤骨髓瘤细胞的能力。结果与单一应用IL-18相比,低浓度IL-2和IL-18联合应用显著提高了NK细胞的活性、分泌干扰素(IFN-γ)的水平和杀伤骨髓瘤细胞的作用。结论低浓度IL-2具有协同IL-18活化NK细胞抗骨髓瘤细胞的作用,此结果为IL-2和IL-18联合应用治疗骨髓瘤奠定了一定的理论基础。

The effect of interleukin-2 (IL-2) synergized with IL-18 to enhance activities of natural killer cells

Objective To explore potential synergetic effects of interleukin-2(IL-2) with IL-18 on induction of natural killer(NK) cell activity against myeloma cells in vitro.Methods CD69 expression on NK cells were defined by Flow Cytometry;IFN-γ level in supernatants of NK cell culture was measured by ELISA;pure NK cell population was obtained from peripheral blood by using cell purification kit;standard Cr51 release method was used to test NK cell cytotoxicity against myeloma cells in vitro.Results The combined application of IL-18 with low dose of IL-2(10u/mL) significantly enhanced NK cell activation,IFN-γ secretion and NK cell cytolytic activity against myeloma cells(U266 and RPMI-8226) as compared to IL-18 alone,respectively.Conclusion IL-2 synergized with IL-18 to induce NK cells activates anti-myeloma cell in vitro and combined use of IL-18 and low dose IL-2 could be an alterative immuno-therapy approach for cancers including myeloma.