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| 人神经生长因子的基因克隆和序列分析 | |
| 引用本文： | 马巍,吴玲,刘淼,任惠民,扬广笑,王全颖,王德利. 人神经生长因子的基因克隆和序列分析[J]. 西安交通大学学报(医学版), 2002, 23(4): 338-341 |
| 作者姓名： | 马巍 吴玲 刘淼 任惠民 扬广笑 王全颖 王德利 |
| 作者单位： | 1. 西安交通大学第一医院骨科 2. 西安交通大学医学院人体解剖学教研室,西安,710061 3. 西安华广生物工程有限公司,西安,710025 |
| 摘 要： | 目的 克隆人神经生长因子基因编码区。方法 由人末梢血白细胞中提取细胞总DNA ,利用PCR法 ,从DNA中扩增出人神经生长因子编码区基因DNA片段 ;将获得的基因片段插入 pGEM T Easy质粒中 ,转化到大肠杆菌DH5α后挑选阳性克隆 ,利用限制性内切酶酶切核苷酸序列分析技术鉴定重组质粒。结果 经质粒DNA酶切分析及序列测定 ,获得了人神经生长因子DNA片段序列。结论 首次由人白细胞DNA中克隆获得了人神经生长因子基因 ,为神经生长因子的基因治疗提供了前提条件。 |
| 关 键 词： | 神经生长因子 聚合酶链反应 T载体 基因克隆 |
| 文章编号： | 1671-8259(2002)04-0338-04 |
| 修稿时间： | 2002-01-24 |
Cloning and sequencing the human NGF gene |
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| Ma Wei,Wu Ling,Liu Miao,Ren Huimin,Yang Guangxiao,Wang Quanying,Wang Deli. Cloning and sequencing the human NGF gene[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2002, 23(4): 338-341 | |
| Authors: | Ma Wei Wu Ling Liu Miao Ren Huimin Yang Guangxiao Wang Quanying Wang Deli |
| Abstract: | Objective Molecular cloning and sequencing of the human nerve growth factor gene. Methods By extracting the human genomic DNA from the white blood cells as templates, the gene of NGF was cloned by PCR and T vector cloning method. The positive clones were identified by the restriction enzymes, and then the amplified cloning was sequenced and analyzed. Results The comparison of the cloned gene of NGF with the GenBank sequence(V01511) showed that both of the sequences were identical and of 353bp length. Conclusion Cloning the NGF gene from the human genomic DNA has paved the way for further study on gene therapy of nervous system damage. |
| Keywords: | nerve growth factor (NGF) polymerize chain reaction (PCR) T vector gene clone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |