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大脑皮层神经细胞原代培养及其缺氧性损害模型
引用本文:刘小红,陈征起,和光祖.大脑皮层神经细胞原代培养及其缺氧性损害模型[J].西安交通大学学报(医学版),1996(1).
作者姓名:刘小红  陈征起  和光祖
作者单位:西安第二附属医院小儿科
摘    要:用2d龄的SD大鼠大脑皮层进行分散神经细胞原代培养,经氰化钠阻断细胞呼吸链,模拟了大脑皮层神经细胞急性缺氧损害模型。模型中缺氧细胞形态不典型,膜流动性降低,乳酸脱氢酶漏出增多,类似整体动物脑缺氧后神经细胞变化。此模型可用于研究缺氧皮层神经细胞的损伤、损伤后恢复及药物的保护作用。

关 键 词:神经细胞  原代培养  缺氧损害  模型

PRIMARY CULTURE OF RAT CEREBRAL CORTICAL NEURONS AND HYPOXIC NEURONS INJURY MODEL IN VITRO
Liu Xiaohong,Chen Zhengqi,He Guangzu.PRIMARY CULTURE OF RAT CEREBRAL CORTICAL NEURONS AND HYPOXIC NEURONS INJURY MODEL IN VITRO[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1996(1).
Authors:Liu Xiaohong  Chen Zhengqi  He Guangzu
Abstract:Primary culture of rat cerebral cortical neurons was prepared from cortices of 2-day old rats and an attempt was made to imitate the hypoxic neurons injury. Hypoxia was induced by adding 1 mmol/L sodium cyanide to the incubation medium of the neurons. In this model, the hypoxic neurons showed untypical morphology, decrease of the neuron membrane fluidity and increase of amounts of LDH leaking out which were similar to the changes in vivo. The results suggested that hypoxic cultured neurons could be a useful model for researching hypoxic neurons and recovering them and testing cerebroprotective drug effect.
Keywords:neuron  primary culture  hypoxic injury  model  
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