补硒治疗慢性肝病患者PBMC功能紊乱的临床价值

目的　探讨应用口服补硒方法纠正慢性肝病患者外周血单个核细胞 (PBMC)免疫功能紊乱的临床价值。方法　患者口服补硒 (2 0 0～ 30 0 μg·d-1) 8～ 12周后分离PBMC ,分别对比观察硒作用前后PBMC膜流动性、白细胞介素 2受体 (IL 2R)表达及其分泌IL 2活性、过氧化脂质丙二醛 (MDA)含量的变化 ,并结合体外PBMC培养实验结果 ,探讨其可能机制。结果　口服补硒治疗后 ,患者PBMC膜流动性、IL 2R表达及其分泌IL 2活性均显著提高 ,血中LPO含量显著减少 ,与未补硒组比较差别均有显著性意义 (P <0 .0 5 ;P <0 .0 1)。体外培养患者的PBMC外加硒作用后上述指标呈相似变化。结论　口服补硒可纠正慢性肝病患者PBMCIL 2系统的功能紊乱 ,其机制可能与抑制PBMC膜的脂质过氧化损伤 ,维护膜的流动性有关

The changes and significance of peripheral blood mononuclear cell membrane fluidity, interleukin-2 production and interleukin-2 receptor expression in chronic hepatitis patients supplemented with selenium

Objective To study the effects of selenium on the peripheral blood mononuclear cells (PBMC) membrane fluidity and the function of patients with chronic hepatitis. Methods PBMC pretreated with selenium (1.156×10 -7mol·L -1) for 6 hours in vivo or extracted directly from the patients after taking Se-yeast continuously for 8～12 weeks (200 μg of selenium one day) were exposed to Con-A for 48 hours. The membrane fluidity, interleukin-2 (IL-2) productionand interleukin-2 receptor (IL-2R) expression of PBMC and the malondialdehyde (MDA) concentration in medium and plasma were determined. Results The PBMC membrane fluidity, IL-2 production and IL-2R expression of the patients with chronic hepatitis were significantly decreased and the MDA concentration in medium or plasma were significantly increased (P<0.01). Both in vivo and vitro,administration of selenium could reverse the parameters above (P<0.05).Conclusion Supplementing selenium can suppress the membrane lipid peroxidation of PBMC, maintain the membrane fluidity and improve the PBMC function of patients with chronic hepatitis.