| DETERMINATION OF POLYDATIN IN POLYGONUM CUSPIDTUM SIEB. ETZUCC. BY TLC-FLUORESCENCE SPECTROPHOTOMETRY |
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| 作者姓名： | 许昭 唐玉海 李健军 杨云 |
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| 作者单位： | DepartmentofChemistry,Xi＇anJiaotongUniversity,Xi＇an710061,China |
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| 摘 要： | Objective A method of TLC-fluorescence spectrophotometry was established to assay the content of polydatin in polygonum cuspidatum sieb. et zucc. Methods: Polydatin was extracted by methanol and separated with ch‘loroform-acetone-formic acid-water (4:4:0.5:0.2) by thin layer chromatography. The excitation wavelength and emission wavelength were 284 nm and 384 nm, respectively. Results The linear regression equation of the calibrationgraph was y=7.02179x 4.5143, a linear regression correlative coefficient r= 0. 9936. Conclusion This method was proved simple, stable and sensitive. It can be used in quality control of herbs.
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| 关 键 词： | 中草药分析 蓼属植物 虎杖苷 线性衰减相关系数 稳定性 过敏性 |
DETERMINATION OF POLYDATIN IN POLYGONUM CUSPIDTUM SIEB.ET ZUCC.BY TLC-FLUORESCENCE SPECTROPHOTOMETRY |
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| Xu Zhao,Tang Yuhai,Li Jianjun,Yang Yun.DETERMINATION OF POLYDATIN IN POLYGONUM CUSPIDTUM SIEB. ETZUCC. BY TLC-FLUORESCENCE SPECTROPHOTOMETRY[J].Academic Journal of Xi’an Jiaotong University,2004,16(1):85-87. |
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| Authors: | Xu Zhao Tang Yuhai Li Jianjun Yang Yun |
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| Institution: | Department of Chemistry, Xi'an Jiaotong University, Xi'an 710061, China |
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| Abstract: | Objective A method of TLC-fluorescence spectrophotometry was established to assay the content of polydatin in polygonum cuspidatum sieb. et zucc. Methods: Polydatin was extracted by methanol and separated with chloroform-acetone-formic acid-water (4∶4∶0.5∶0.2) by thin layer chromatography. The excitation wavelength and emission wavelength were 284nm and 384nm, respectively. Results The linear regression equation of the calibration graph was y=7.02179x+4.5143, a linear regression correlative coefficient r=0.9936. Conclusion This method was proved simple, stable and sensitive. It can be used in quality control of herbs. |
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| Keywords: | TLC-fluorescence spectrophotometry polydatin polygonum cuspidatum sieb et zucc |
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