| IgM抗体捕获ELISA法对流行性出血热患者尿液中特异性IgM抗体的检测 |
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| 引用本文： | 刘正稳,李秀如,张成文,岳金声,吴素歌.IgM抗体捕获ELISA法对流行性出血热患者尿液中特异性IgM抗体的检测[J].西安交通大学学报(医学版),1990(2). |
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| 作者姓名： | 刘正稳 李秀如 张成文 岳金声 吴素歌 |
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| 作者单位： | 西安医科大学第一附属医院传梁科
(刘正稳，李秀如，张成文，岳金声)，西安医科大学第一附属医院传梁科(吴素歌) |
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| 摘 要： | 将IgM抗体捕获ELISA法应用于检测流行性出血热病人尿液中的特异性IgM抗体,总阳性率72.62%,7～9病日可达83.87%;第3病日即可出现阳性;并且具有较高的特异性和敏感性。尿中特异性IgM抗体检出的阳性率与病日(G=7.3858,P>0.05)及尿蛋白含量(G=4.4003,P>0.05)间均无明显关系;特异性IgM抗体的滴度与病日有关(F′=26.5331,P<0.01)与尿蛋白含量无明显关系(F=2.2074,P>0.05);不同病日尿液中特异性IgM抗体检出的阳性率与滴度呈正相关(r=0.9973,P<0.01)。
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| 关 键 词： | 出血热 流行性 酶连接免疫吸附测定 尿 免疫球蛋白M类 |
DETECTION OF URINARY SPECIFIC IGM ANTIBODIES IN EPIDEMIC HEMORRHAGIC FEVER BY IGM ANTIBODY CAPTURE ELISA TECHNIQUE |
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| Liu Zhengwen,et al.DETECTION OF URINARY SPECIFIC IGM ANTIBODIES IN EPIDEMIC HEMORRHAGIC FEVER BY IGM ANTIBODY CAPTURE ELISA TECHNIQUE[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1990(2). |
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| Authors: | Liu Zhengwen |
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| Abstract: | The specific IgM(SIgM) antibodies in 84 urine specimens from 52 patients with epidemic hemorrhagie fever (EHF) were detected by an IgM antibody capture ELISA technique with mixed monoclonal antibodies against multiple EHF virus strains. The total positive rate and the positive rate from day 7 to day 9 of illness were 72.62% and 83.87% respectively. The SIgM positive urine specimens oecured on day 3 of illness. The urine specimen, from healthy persons and the persons with other diseases were all negative. Therefore, high specificity and sensitivity were observed. No significant correlations were found both between the positive rates and drays of illness and between p(?)sitive rates and protein levels in urine specimens. The SIgM antibody titres were related with the days of illness rather than the protein levels in urine specimens. Furthermore, the positive rates were increased positively with the SIgM antibody titres. |
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| Keywords: | hemorrhagic fever epidemic ELISA IgM urine |
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