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| 海藻酸盐小球中培养的软骨细胞的生长 | |
| 引用本文： | 任利玲,冯雪,马东洋,王建卫,陈富林,丁寅.海藻酸盐小球中培养的软骨细胞的生长[J].西安交通大学学报(医学版),2011,32(1):57-61. |
| 作者姓名： | 任利玲 冯雪 马东洋 王建卫 陈富林 丁寅 |
| 作者单位： | 1. 兰州大学口腔医学院正畸科,甘肃兰州,730000 2. 第四军医大学口腔医院正畸科,陕西西安,710032 3. 兰州军区总医院口腔颌面外科,甘肃兰州,730052 4. 西北大学生命科学院组织工程中心,陕西西安,710000 |
| 基金项目： | 国家自然科学基金资助项目，兰州大学中央高校基本科研业务费专项资金资助项目 |
| 摘 要： | 目的为海藻酸盐水凝胶作为软骨细胞三维培养环境提供实验依据。方法将酶消化法获取的P1代软骨细胞包裹在20 g/L海藻酸钙凝胶小球中持续培养4周,在培养的不同时间点取适量的小球行倒置相差显微镜观察,HE、番红"O"染色及Ⅱ型胶原免疫组化染色;并通过MTT法检测细胞活性及增殖;二甲基亚甲兰法进行氨基葡聚糖(GAG)含量测定。结果在海藻酸钙小球内培养的软骨细胞能够保持球形状态,且随时间的延长,细胞增殖逐渐聚集成簇;细胞外基质沉积逐渐增加,GAG含量增多。Ⅱ型胶原免疫组化染色阳性,鉴定证实为具有正常表型的软骨细胞。结论软骨细胞在海藻酸盐凝胶小球中培养有效维持了细胞的分化状态,细胞增殖并具有良好的分泌蛋白功能。海藻酸钙凝胶适合于软骨细胞的生长。 |
| 关 键 词： | 软骨细胞 海藻酸盐 三维培养 细胞培养 |
Biological response of chondrocytes encapsulated in alginate beads |
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| REN Li-ling,FENG Xue,MA Dong-yang,WANG Jian-wei,CHEN Fu-lin,DING Yin.Biological response of chondrocytes encapsulated in alginate beads[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(1):57-61. | |
| Authors: | REN Li-ling FENG Xue MA Dong-yang WANG Jian-wei CHEN Fu-lin DING Yin |
| Institution: | REN Li-ling1,FENG Xue2,MA Dong-yang3,WANG Jian-wei1,CHEN Fu-lin4,DING Yin2(1.Department of Orthodontics,School of Stomatology,Lanzhou University,Lanzhou 730000,2.Department of Orthodontics,Fourth MilitaryMedical University,Xi'an 710032,3.Department of Oral and Maxillofacial Surgery,Lanzhou General Hospital of Lanzhou Command of Chinese People's Liberation Army,Lanzhou 730052,4.Rege Lab of Tissue Engineering,Department of Bioscience,Faculty of Life Science,Northwest University,Xi'an 710... |
| Abstract: | Objective To explore the possibility of alginate as a three-dimensional matrix for chondrocytes in vitro.Methods Rabbit chondrocytes were isolated from the articular cartilage of a 4-week-old rabbit by enzyme digestion method.P1 chondrocytes suspended in calcium alginate beads were observed dynamically under phrase contrast microscope.The viability and proliferation of cells were evaluated by MTT,the quantity of glycosaminoglycan(GAG) was determined by a modified dimethyl-methylene blue method,histological ... |
| Keywords: | chondrocyte alginate three-dimensional culture cell culture |
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