| NK细胞杀伤ARH-77细胞的活性及机制的初步探讨 |
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| 引用本文： | 周健,宋永平,魏旭东,郭坤元,孙明. NK细胞杀伤ARH-77细胞的活性及机制的初步探讨[J]. 西安交通大学学报(医学版), 2011, 32(1): 97-101 |
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| 作者姓名： | 周健 宋永平 魏旭东 郭坤元 孙明 |
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| 作者单位： | 1. 河南省肿瘤医院血液科、河南省血液病研究所,河南郑州,450003
2. 南方医科大学珠江医院血液科,广东广州,510282 |
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| 摘 要： | 目的探讨同种异体NK细胞杀伤人骨髓瘤ARH-77细胞的活性及机制。方法 LDH释放法检测NK细胞杀伤ARH-77细胞的活性;RT-PCR方法和流式细胞仪分别检测K562和ARH-77细胞NKG2D配体和HLA-I基因和分子。阻断K562和ARH-77细胞NKG2D配体,观察NK细胞杀伤活性的变化。结果相同效靶比时NK细胞杀伤ARH-77细胞的活性明显低于杀伤K562细胞。两种细胞均表达MICA/B和ULBP1～3基因。K562细胞高表达MICA/B和ULBP1～3分子,不表达HLA-Ⅰ类分子;ARH-77细胞低表达ULBP1～3分子,高表达HLA-Ⅰ类分子。ARH-77细胞HLA基因型为A2、3,B15、35,Cw3、4。阻断NKG2D配体,NK细胞对K562细胞的杀伤活性明显降低,对ARH-77细胞的杀伤活性无明显改变;阻断HLA-Ⅰ类分子后,NK细胞对K562细胞的杀伤活性无变化,对ARH-77细胞的杀伤活性明显提高。结论 ARH-77细胞对NK细胞的杀伤敏感性低,其机制与其高表达HLA-Ⅰ类分子、低表达NKG2D配体有关。
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| 关 键 词： | 自然杀伤细胞 自然杀伤细胞受体 骨髓瘤 HLA-Ⅰ类分子 |
Cytotoxicity of allogenic NK cells against myeloma cells in vitro and its mechanism |
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| ZHOU Jian,SONG Yong-ping,WEI Xu-dong,GUO Kun-yuan,SUN Ming. Cytotoxicity of allogenic NK cells against myeloma cells in vitro and its mechanism[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2011, 32(1): 97-101 |
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| Authors: | ZHOU Jian SONG Yong-ping WEI Xu-dong GUO Kun-yuan SUN Ming |
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| Affiliation: | ZHOU Jian1,SONG Yong-ping1,WEI Xu-dong1,GUO Kun-yuan2,SUN Ming2(1.Department of Hematology,Henan Tumor Hospital/Henan Institute ofHematology,Zhengzhou 450003,2.Department of Hematology,Zhujiang Hospital of Southern Medical University,Guangzhou 510282,China) |
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| Abstract: | Objective To observe the cytotoxicity of allogenic natural killer(NK) cells against myeloma cells in vitro and preliminarily explore its molecular mechanism.Methods Cytotoxicity of NK cells against ARH-77 cells was analyzed by LDH releasing assay at different effector-to-target cell ratios(E:T).The genes and proteins of NKG2D ligands on K562 and ARH-77 cell surface were measured by RT-PCR and flow cytometry,respectively.The genes of HLA-I molecules in ARH-77 cell and KIR of NK cells were assayed by PCR-SSCP... |
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| Keywords: | natural killer(NK) cell NK cell receptor myeloma HLA-I molecule |
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