| 人颈椎间盘退变基因表达谱的研究 |
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| 引用本文： | 张小卫,郭团茂,刘淼,张银刚.人颈椎间盘退变基因表达谱的研究[J].西安交通大学学报(医学版),2011,32(3):340-343,347. |
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| 作者姓名： | 张小卫 郭团茂 刘淼 张银刚 |
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| 作者单位： | 西安交通大学医学院第一附属医院骨科,陕西西安,710061 |
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| 摘 要： | 目的探讨人的颈椎间盘组织基因表达谱的变化,分析椎间盘退变的发生机制。方法改进的一步法抽取6例退变和6例正常颈椎间盘髓核的总RNA,用Cy3、Cy5荧光标记制成cDNA探针,再与22 575个cDNA基因表达芯片杂交,激光扫描荧光信号,对差异基因表达谱进行分析。结果进行6次扫描,退变和正常椎间盘髓核组织共有570个基因发生了差异表达,其中上调550个,下调20个。结论多种基因表达异常与颈椎间盘退变相关。
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| 关 键 词： | 颈椎间盘 退变 基因芯片 基因表达谱 差异表达 |
Gene expression pattern of cervical disc degeneration of humans |
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| ZHANG Xiao-wei,GUO Tuan-mao,LIU Miao,ZHANG Yin-gang.Gene expression pattern of cervical disc degeneration of humans[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(3):340-343,347. |
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| Authors: | ZHANG Xiao-wei GUO Tuan-mao LIU Miao ZHANG Yin-gang |
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| Institution: | ZHANG Xiao-wei,GUO Tuan-mao,LIU Miao,ZHANG Yin-gang (Department of Orthopedics,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China) |
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| Abstract: | Objective To investigate the gene expression changes in cervical intervertebral disc of humans and analyze the mechanism of disc degeneration.Methods The total RNAs were isolated by improved one-step method from human cervical disc of both 6 cases of degeneration and 6 normal ones.The transcribed cDNAs were labeled with the incorporations of fluorescent dNTP(Cy3,Cy5) for the probes.The cDNA microarray was scanned for the fluorescent signals by laser scanner and analyzed by computer image analysis after the ... |
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| Keywords: | cervical disc degeneration DNA microarray gene expression pattern differential expression |
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