脂质体技术促进c-myc反义寡核苷酸导入人体肝癌细胞的作用 |
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引用本文: | 程延安,党双锁,吴军正,曾嵘. 脂质体技术促进c-myc反义寡核苷酸导入人体肝癌细胞的作用[J]. 西安交通大学学报(医学版), 2000, 21(4): 367-368 |
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作者姓名: | 程延安 党双锁 吴军正 曾嵘 |
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作者单位: | 1. 西安交通大学第二医院肝胆病医院,西安 710004 2. 第四军医大学口腔生物学教研室 |
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摘 要: | 观察脂质体技术促进 c- myc反义寡核苷酸导入人体肝癌细胞的作用。方法 采用新一代脂质体 Lipofect AMINE( LR)包裹针对肝癌表达基因 c- myc反义寡核苷酸 ( ASODN) ,借助FITC荧光标记 c- myc- ASODN,应用细胞培养方法及荧光分光光度计以及荧光显微镜。结果 LR促进 ASODN进入靶细胞并增强其对肿瘤细胞基因的特异性封闭作用。结论 脂质体作为一种安全、简便、有效的基因转移载体广泛适用医学科研
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关 键 词: | 脂质体 肝肿瘤 基因治疗 |
文章编号: | 0258-0659(2000)04-0367-02 |
修稿时间: | 2000-03-24 |
Enhancement of transfection of c-myc antisense oligodeoxynucleotide in hepatoma cell through lipof ectAMINE |
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Abstract: | Objective To observe the effect of LR in enhancing the transfection of c myc ASODN in liver cells.Methods A new lipofect AMINE (LR) was used entraping c myc antisense oligodeoxynucleotide(ASODN) of hepatoma.Through labeling c myc ASODN with FITC, cell culture ,fluorecence spectrophotometer, and fluorecence mircoscope were used.Results LR was able to enhance the c myc ASODN transfection into hepatoma cell ,and to strengthen the blockage to the specificity of hepatoma cell genes.Conclusion LR may be used widely in research and work as a safe, convenient, effective gene transfering carrier. |
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Keywords: | hapatoma lipofect AMINE gene therapy |
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