| 截短血小板生成素cDNA在中华仓鼠卵巢细胞中的表达 |
| |
| 引用本文： | 王志云,田淑芳,楚雍烈,阮力. 截短血小板生成素cDNA在中华仓鼠卵巢细胞中的表达[J]. 西安交通大学学报(医学版), 2001, 22(6): 510-513 |
| |
| 作者姓名： | 王志云 田淑芳 楚雍烈 阮力 |
| |
| 作者单位： | 1. 西安交通大学微生物学教研室,
2. 中国预防医学科学院病毒学研究所, |
| |
| 摘 要： | 目的 将具有全长血小板生成素 (TPO)活性的N端截短血小板生成素cDNA(T1 84)作为目的基因 ,在中华仓鼠卵巢细胞 (CHO)中成功表达。方法 将N端截短血小板生成素cDNA克隆入以二氢叶酸还原酶 (DHFR)为筛选扩增基因的 pDC B真核表达载体 ,通过不断提高MTX浓度 ,促进N端截短血小板生成素在CHO细胞中的表达。结果 构建了N端截短血小板生成素cDNA的真核表达质粒 ,并能在CHO细胞中高效表达。结论 N端截短血小板生成素cDNA在CHO细胞中成功表达 ,其产物具有全长TPO的活性。
|
| 关 键 词： | 血小板生成素 中华仓鼠卵巢细胞 基因表达 |
| 文章编号： | 0258-0659(2001)06-0510-04 |
| 修稿时间： | 2001-11-01 |
Expression of N-terminal truncated hTPO in CHO cell |
| |
| Wang Zhiyun,Tian Shufang,Chu Yonglie,et al. Expression of N-terminal truncated hTPO in CHO cell[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2001, 22(6): 510-513 |
| |
| Authors: | Wang Zhiyun Tian Shufang Chu Yonglie et al |
| |
| Affiliation: | Wang Zhiyun,Tian Shufang,Chu Yonglie,et al Department of Microbiology,Medical School of Xi'an Jiaotong University,Xi'an 710061,China |
| |
| Abstract: | Objective To obtain N-terminal truncated hTPO (T 184 ) in CHO cell. Methods T 184 cDNA was obtained by PCR from plasmid pUC18-TPO, then was digested with restricted enzyme NotⅠ, big fragment was recovered from pDC-β which also digested with NotⅠ,T 184 cDNA was linked with the big fragment of pDC-β.The recombinant vector pDC-T 184 was transfected into CHO DHFR-cell. After selected and amplified with MTX, N-terminal truncated hTPO (T 184 ) was expressed in CHO cell.Results The recombinant plasmid pDC-T 184 was constructed and could express T 184 protein efficiently.Conclusion N-terminal truncated hTPO (T 184 ) cDNA has expressed successfully in CHO cell. |
| |
| Keywords: | thrombopoietin chinese hamster ovary cell gene expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
