大鼠Barrett食管癌变相关基因的初步筛选

目的初步筛选大鼠Barrett食管(Barrettsesophagus,BE)癌变相关基因。方法建立胃十二指肠食管反流SD大鼠动物模型,用含有4096条双点大鼠cDNA芯片分别比较BE、食管腺癌(esophagealadenocarcinoma,EA)与正常食管上皮(normalcontrol,NC)mRNA差异表达情况。结果芯片杂交差异表达在3倍以上的基因:EA和NC杂交芯片377项,其中上调表达90条,下调表达142条;BE和NC杂交芯片448项,其中上调表达312条,下调表达136条。相对于BE,EA表达上调的基因112条,下调156条。与肿瘤发生相关的已知基因24条,上调18条,下调6条。结论与BE比较,EA基因表达谱发生了明显改变,与肿瘤发生相关的基因改变明显,差异表达的基因可能与肿瘤的发生相关。

Initially screening genes related to tumorigenesis of Barrett''''s esophagus in rats

Objective To initially screen genes related to tumorigenesis of Barrett's esophagus(BE) in rats. Methods Eight-week-old SD rat models were established to produce gastroduodeno-esophageal reflux, and a group of rats with sham operation were chosen as controls. Esophageal epithelium of pathological tissues were dissected in liquid nitrogen immediately according to pathological classification 40 weeks after surgery. The profiles expressed of 4 096 genes in EA and BE tissues were compared with those in normal esophagus epithelium (NC) by cDNA microarray. Results A total of 448 genes in BE were more than 3 times different from those in NC, including 312 up-regulated and 136 down-regulated genes, and among those 377 genes in EA, 90 were up-regulated and 142 were down-regulated. Compared with BE, 112 genes were up-regulated and 156 down-regulated in EA. The 18 up-regulated genes, whose function was partly known, were involved in carcinogenesis, while 6 down-regulated genes, whose function was clear in some aspect, were involved in carcinogenesis. Conclusion Esophageal epithelium exposed excessively to harmful ingredients of duodenal and gastric reflux could gradually lead to esophagitis, BE and EA. Gene expression level is different between EA and BE, which might be related to the development and progression of EA.