| 用PCR技术标记双链DNA探针 |
| |
| 引用本文： | 宋建明,孙毅,杨居祥,司履生.用PCR技术标记双链DNA探针[J].西安交通大学学报(医学版),1996(4). |
| |
| 作者姓名： | 宋建明 孙毅 杨居祥 司履生 |
| |
| 作者单位： | Department of Immunopathology，Xi'an 710061 |
| |
| 摘 要： | 利用ＰＣＲ技术制备生物素或地高辛标记的ＤＮＡ探针，经原位杂交试验及敏感性和特异性检测，均取得满意效果。ＰＣＲ标记法具有经济、快速、简易和标记量大等优点。实践中发现Ｂｉｏ／Ｄｉｇ－１１－ｄＵＴＰ的质量、ｄＴＴＰ和Ｂｉｏ／Ｄｉｇ－１１－ｄＵＴＰ的比例及起始ＤＮＡ模板浓度是标记成败的关键。
|
| 关 键 词： | 聚合酶链反应 探针标记 核酸杂交 |
DSDNA PROBES LABELLED WITH PCR TECHNIQUE |
| |
| Song Jianming, Sun Yi, Yang Juxiang,et al.DSDNA PROBES LABELLED WITH PCR TECHNIQUE[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1996(4). |
| |
| Authors: | Song Jianming Sun Yi Yang Juxiang |
| |
| Abstract: | Using PCR technique, dsDNAprobes were prepared with incorporating Bio-11-dUTP or Dig-11-dUTP. satisfied effects have been achieved by hybridizing in situ and detecting their sensitivity and specificity. The PCR labelling method characteristically possesses many advantages, such as economy, quickness, easiness and large amounts of labelling, etc. In practice, it has been proved that the keys to the labelling success and failure are the quality of Bio/Dig-11-dUTP, the ratio of dTTP and Bio/Dig-11-dUTP as well as the concentration of initiating template DNA. |
| |
| Keywords: | polymerase chain reaction probe labelling nucleic acid hybridization |
| 本文献已被 CNKI 等数据库收录！ |
|
