α-干扰素对慢性粒细胞白血病来源的树突状细胞的免疫调节作用(Ⅰ)

目的　于慢性粒细胞白血病 (CML)来源的树突状细胞 (DCs)无血清培养基中 ,加入α 干扰素 (IFN α) ,以探讨IFN α对CML DCs表达共刺激分子及分泌IL 10、IL 12P70的影响。方法　在诱导CML DCs的培养基中 ,除加入SCF、GM CSF、TNF α及IL 4外 ,还加入不同浓度IFN α。培养 10～ 14d后 ,流式细胞仪检测细胞免疫表型 ;G显带法显示Ph1染色体 ;噻唑蓝 (MTT)法检测CML DCs刺激正常人外周血淋巴细胞增殖状况 ;ELISA法检测培养上清IL 10及IL 12P70含量。结果　IFN α(30 0U·mL-1)组较无IFN α组 ,CML DCs的CD4 0、CD83、CD86及MHC I类抗原的表达均升高一倍 ;异体混合淋巴细胞反应 (MLR)中OD值增加一倍 ;Ph1染色体阳性比例、IL 10及IL 12P70浓度均减低。结论　IFN α能够部分纠正CML DCs免疫表型及功能缺陷 ,同其解除了CML血清中IL 10对CML DCs分化的抑制有关

Immunoregulatory effects of interferon-α on dendritic cells from patients with chronic myeloid leukemia (I)

Objective To explore the effects of interferon-α(IFN-α) on expressions of costimulatory molecules and secretions of interleukin-10 (IL-10) and IL-12 P70 in dendritic cells (DCs) of chronic myeloid leukemia (CML). Methods In addition to adding stem cell factor (SCF), granulocyte-macrophage-colony-stimulating factor (GM-CSF), tumor necrosis factor-α(TNF-α) and IL-4, IFN-α was added to the serum-free medium of DCs. After 10～14ds' culture, phenotypes and function of CML-DCs were evaluated, respectively, by flow cytometry and methyl thiazolyl tetrazolium (MTT) assay. Chromosome analysis of DCs was made by displaying G banding assays. The concentrations of IL-10 and IL-12P70 in supernatants were evaluated by enzyme-linked immunosorbent assay (ELISA). Results Expressions of CD40, CD83, CD86 and major histocompatibility complex (MHC)-I antigen molecules and the OD number in allogeneic mixed-lymphocyte reaction (MLR) in IFN-α(300U·mL -1) group were twice as high as those in non- IFN-α group. The percentage of Ph 1positive cells and concentrations of IL-10 and IL-12 P70 were reduced in IFN-α group. Conclusion Defective phenotypes and functions of CML-DCs can be corrected partly by IFN-α. The mechanism may lie in the fact that the inhibitory effect of IL-10 on CML-DCs is relieved partly through the regulation of IFN-α.