| TNF-α基因表达在大鼠急性重症胰腺炎肺损伤中的作用 |
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| 引用本文： | 徐军,张梅,刘学民,潘承恩,刘青光. TNF-α基因表达在大鼠急性重症胰腺炎肺损伤中的作用[J]. 西安交通大学学报(医学版), 2004, 25(4): 388-389,392 |
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| 作者姓名： | 徐军 张梅 刘学民 潘承恩 刘青光 |
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| 作者单位： | 西安交通大学第一医院肝胆外科,陕西西安,710061 |
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| 基金项目： | 国家自然科学基金资助 (No .30 371 398) |
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| 摘 要： | 目的 探讨急性重症胰腺炎 (ASP)大鼠肺组织肿瘤坏死因子 α(TNF α)基因表达在肺损伤中所起的作用。方法 将ASP大鼠分别在 1、3、5、7、9h点剖杀 ,取肺组织行病理观察、微血管通透性、组织含水量及髓过氧化物酶(MPO)活性测定 ,RT PCR法检测肺组织TNF α基因表达。结果 制模后肺组织TNF α基因表达在 1h后逐渐上升 ,至9h达到峰值 (P <0 .0 5 )。同时肺损伤程度逐渐加重 ,肺组织微血管通透性、组织含水量及MPO活性逐渐升高。结论 ASP大鼠肺组织TNF α基因过度表达 ,且与肺损伤严重程度相关
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| 关 键 词： | 重症胰腺炎 急性肺损伤 肿瘤坏死因子 大鼠 |
| 文章编号： | 1671-8259(2004)04-0388-02 |
Intrapulmonary expression of TNF-α gene in rats with acute severe pancreatitis |
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| Xu Jun,Zhang Mei,Liu Xuemin,Pan Cheng''''en,Liu Qingguang. Intrapulmonary expression of TNF-α gene in rats with acute severe pancreatitis[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2004, 25(4): 388-389,392 |
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| Authors: | Xu Jun Zhang Mei Liu Xuemin Pan Cheng''''en Liu Qingguang |
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| Abstract: | Objective To study the relationship between the intrapulmonary expression of TNF-α gene and acute lung injury in acute severe pancreatitis (ASP). Methods The rats were sacrificed at 1, 3, 5, 7 and 9 hours after inducing model. Lung tissues were obtained for pathological study, microvascular permeability, dry/wet ratio and MPO examination. Gene expression of TNF-α in lung tissues was detected by RT-PCR. Results After ASP model induction, gene expression of TNF-α in the lung rose gradually after 1 hour and reached peak at 9 hours (P<0.05), accompanied with gradual increase of microvascular permeability, dry/wet ratio and MPO activity. Conclusion Severity of lung injury can be predicted by the gene expression of TNF-α in ASP. |
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| Keywords: | severe pancreatitis acute lung injury tumor necrosis factor rat |
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