浙贝母鳞茎愈伤组织的诱导及植株再生

比较了不同的基本培养基、激素以及激素组合对诱导浙贝母愈伤组织和原球茎的影响．浙贝母愈伤组织和原球茎的诱导及分化与培养基、激素组合和外植体的类型有关．在MS 1．0mg／L2，4-D 1．5mg／L NAA 300mg／L水解酪蛋白 0．55％(质量分数)琼脂培养基中，以浙贝母鳞茎作为外植体诱导愈伤组织的结果最好．在MS 1．0mg／L NAA 2．0mg／L ZT 0．55％琼脂培养基中，从浙贝母鳞茎黄褐色愈伤组织上诱导再生植株效果最好．诱导原球茎最好的培养基是MS 1．0mg／L 2，4-D 2．0mg／L KT 0．55％琼脂。

Callus Induction and Plant Regeneration from Bulbs of Fritillaria thunbergii Miq.

The effects of mediums, phytohormones and phytohormone combinations on induction of calluses and protocorm-like bodies (PLBs) from the explants of bulbs of Fritillaria thunbergii Miq.. The induction of calluses and PLBs is closely related to medium, hormone combination and the type of the explants. The best callus induction is achieved in Murashige-Skoog (MS) medium supplemented with 1.0 mg/L 2, 4-D (2,4-dichlorophenoxyacetic acid)+1.5 mg/L NAA (1-naphthalene acetic acid)+300 mg/L casein hydrolysate+0.55% (mass proportion) agar by taking the bulbs of Fritillaria thunbergii Miq. as explants. Plantlets are best regenerated from snuff-colored calluses of the bulbs of Fritillaria thunbergii Miq. in MS medium supplemented with 1.0 mg/L NAA+2.0 mg/L ZT (zeatin)+0.55% casein hydrolysate. The best medium for PLBs induction is MS supplemented with 1.0 mg/L 2,4-D+2.0 mg/L KT (6-furfurylaminopurine)+0.55% casein hydrolysate.