| 大鼠脑发育过程中碱性成纤维细胞生长因子的免疫组织化学研究 |
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| 引用本文： | 肖新莉,刘勇,徐曦,陈新林,丁海燕.大鼠脑发育过程中碱性成纤维细胞生长因子的免疫组织化学研究[J].西安交通大学学报(医学版),2003,24(5):434-437. |
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| 作者姓名： | 肖新莉 刘勇 徐曦 陈新林 丁海燕 |
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| 作者单位： | 1. 西安交通大学医学院人体解剖学教研室,陕西西安,710061
2. 陕西医学高等专科学校人体解剖学教研室,陕西西安,710068 |
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| 基金项目： | 国家自然科学基金资助项目 (No.39740 0 2 8) |
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| 摘 要： | 目的 研究碱性成纤维细胞生长因子 (bFGF)阳性细胞在大鼠不同发育时期不同脑区的分布。方法 应用免疫组织化学ABC法 ,对不同发育时期大鼠脑的嗅球、室管膜、室管膜下区、顶叶皮质、纹状体、海马进行bFGF免疫组织化学染色及光镜观察。结果 bFGF在脑内广泛表达 ,其表达强弱随年龄和部位不同而变化。E18～ 3d组bFGF阳性细胞显色较弱 ,以后各组显色较强。阳性细胞呈现胞浆或 (和 )核阳性。结论 bFGF在脑内分布广泛 ,其对中枢神经系统的发育及生长发挥重要作用
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| 关 键 词： | 碱性成纤维细胞生长因子(bFGF) 脑 大鼠 免疫组织化学 |
| 文章编号： | 1671-8259(2003)05-0434-04 |
| 修稿时间： | 2002年10月16 |
Immunohistochemical study of bFGF in developing brain of rats |
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| Xiao Xinli,Liu Yong,Xu Xi,Chen Xinlin,Ding Haiyan.Immunohistochemical study of bFGF in developing brain of rats[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2003,24(5):434-437. |
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| Authors: | Xiao Xinli Liu Yong Xu Xi Chen Xinlin Ding Haiyan |
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| Abstract: | Objective To observe the distribution of basic fibroblast growth factor (bFGF) positive cells in different cerebral regions during brain development in rat. Methods Immunohistochemical method was used to observe bFGF expression in the olfactory bulb, ependyma, subependymal zone, parietal cortex, striatum and hippocampus in different developing periods of the rat brain. Results bFGF was expressed extensively in the observed brain regions from embryonic to old period. The intensity of bFGF expression was changed with age and cerebral regions. bFGF staining was weak in E18～3d groups and strong in the other groups. The cells were positive in cytoplasm and/or nucleus. Conclusion Distributed extensively in the brain, bFGF may play an important role in the development and growth of the central nervous system. |
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| Keywords: | basic fibroblast growth factor (bFGF) brain rat immunohistochemistry |
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