慢性氟中毒作用下小鼠切牙成釉细胞的改变

目的　通过观察慢性氟中毒作用下小鼠切牙成釉细胞形态学变化 ,探讨氟斑牙的形成机制。方法　建立小鼠氟斑牙动物模型 ,在不同时期肉眼观察成活小鼠切牙颜色及釉质表面变化 ,4 5d后处死动物 ,HE染色 ,光镜观察小鼠切牙成釉细胞各个时期的细胞形态学变化。结果　随着小鼠日常饮用水中氟化钠浓度的增加和时间的延长 ,小鼠切牙氟斑牙症状加重 ;成釉细胞出现扭曲变形、正常的高柱状形态丧失、胞内出现空泡及大小不等的黑色颗粒、托姆斯突被异位物质压迫等细胞形态学改变突出。结论　氟对小鼠切牙的毒性效应在其发育初始阶段即已产生影响 ,并与时间和剂量有密切关系。从小鼠切牙发育初始阶段研究氟斑牙的致病机制可能会有所启示

Changes in ameloblasts of mouse incisor exposed to chronic fluorosis

Objective To investigate changes in ameloblasts of incisor under chronic fluorine poisoning. Methods The mice dental fluorosis model was established. The color and morphologic changes in every stage of mouse incisor were observed with naked eyes. After 45 days the mice were executed and histological changes in ameloblasts of their incisor were observed at different stages. Result With consistent increase in sodium fluoride and time morphological changes in the mice incisor gradually aggravated and the ameloblast distorted and lost its normal column contour, and vacuoles and black granules of various sizes appeared. The Thomas' process was compressed by lateral ectopic material. Conclusion The toxic effect of fluorine on mouse incisor begins to show at early stage of its development, which is closely related with time and dose. The fluorosis mechanism will be enlightened by pathogenesis investigations of fluorosed teeth at the earlier stage of tooth development.