| 白细胞介素2诱生和检测的实验条件分析 |
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| 引用本文： | 陈太平,李义方,蒋平.白细胞介素2诱生和检测的实验条件分析[J].西安交通大学学报(医学版),1989(3). |
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| 作者姓名： | 陈太平 李义方 蒋平 |
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| 作者单位： | 西安医科大学肝炎研究室
(陈太平，李义方)，西安医科大学肝炎研究室(蒋平) |
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| 摘 要： | 本文对人外周血单个核细胞(PBMC)诱生白细胞介素2(IL-2)及用ConA 激活的小鼠脾细胞检测IL-2活性过程中的几个实验条件进行了摸索,发现1×10~6 PBMC/ml 经10ug ConA/ml 刺激48h 为诱生IL-2的最佳条件,检测IL-2活性的最佳条件是:2.5×10~4每孔检测细胞/0.1ml/与等量样品共同培养24h.作者认为,小鼠脾细胞经10ug ConA/ml 剌激48h 后.大部分转化为淋巴母细胞,适用于IL-2活性的检测,并具有一定的特异性和重复性。
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| 关 键 词： | 白细胞介素2 辅助细胞 淋巴激活素 |
OPTIMAL CONDITIONS FOR THE PRODUCTION AND ASSAY OF INTERLEUKIN 2 |
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| Chen Taiping,et al.OPTIMAL CONDITIONS FOR THE PRODUCTION AND ASSAY OF INTERLEUKIN 2[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1989(3). |
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| Authors: | Chen Taiping |
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| Abstract: | Experiments were carried out to obtain optimal conditions for intericukin 2(IL-2)production of human peripheral blood mononuclear cells(PBMCs),and for IL-2 assay by using ConA-stimulatedmurine spleen cells(ConA-SMSCs).Optimal condi-tions for IL-2 production were achieved at a concentra-tion of 1×10~6 PBMCs/ml when stimulated with 10μgConA.IL 2 production was maximal at 48 h andthen gradually declined.As for IL 2 activity assay,maximal growth index(GI)of each sample wasyielded after 24 h of culture of 25×10~4(100 ul)ConA-SMSCs per well in the presence of this sample(100 ul).We hold that this bioassay is specific andreproducible for the detection of IL 2 activity. |
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| Keywords: | interlcukin 2 helper cells lymphokines |
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