雌激素对骨关节炎模型软骨基质蛋白多糖变化的影响

目的　观察雌激素对骨关节炎 (OA)模型软骨基质蛋白多糖变化的影响 ,探讨外源性雌激素治疗OA的作用机理。方法　以家兔制作OA动物模型 ,在雌激素干预治疗的基础上 ,获取病变早、中、晚期关节软骨 ,提取葡糖氨基聚糖 (GAG) ,用生化和放射免疫技术测定其中不同种类GAG及其功能基团的含量。结果　在病变的不同时期 ,对照组GAG的总量、透明质酸的含量均减少 ,硫酸软骨素与硫酸角质素的含量和比例增高 ;而雌激素干预组GAG的总量、透明质酸的含量、硫酸软骨素与硫酸角质素的含量和比例与正常组接近。结论　外源性雌激素通过抑制糖蛋白降解对OA提供保护作用。

Effects of estrogen on the changes of proteoglycan in articular cartilage matrix of rabbits with osteoarthritis

Objective To observe the effects of ectogenetic e strogen on changes of proteoglycan (PG) in articular cartilage matrix of osteoar thritis(OA) and to explore the treatment of ectogenetic estrogen on OA. Methods The rabbit model of OA was prepared, and on the bas is of interveneing therapy, the articular cartilage at the early, middle and lat e stages of OA was obtained. Glycosaminoglycan (GAG) was extracted from cartilag e, and the amount of different kinds of GAG and its function groups were detecte d with biochemical and radioimmunologic techniques. Results The content of total GAG and hayluronic all decline d, but the CS/KS and CS/KS increased. Conclusion Ectogenetic estrogen has function of anti-degra ding PG and offering protection for patients with OA.