200例国人DNA纹印基因频率分布—D2S44及D17S79位点的DNA多态性

DNA 纹印具有高度多态性,是解决个体识别及亲子鉴定等问题的最新技术.本文利用此项技术,研究了200名国人的D2S44及D17S79二位点的等位基因频率分布.结果发现D2S44位点片段带大小变异数为29,其中可检出两个等位基因团.一在7.7和9.1kb 之间,最常见于8.0kb,另一在9.8和14.1kb 之间,最大频率在11.1kb,占17.25%。D17S79位点片段带大小变异数为27,等位基因团在约4kb大小的范围内,最大频率在3.4kb,占29.55%.与国外报道结果相比,显示种族差异.

ALLELE FREQUENCY DISTRIBUTION OF DNA FINGERPRINTS IN 200 CHINESE-DNA POLYMORPHISM AT D2S44 AND D17S79 LOCI

Analysis of DNA fingerprints characterized by theirpolymorphism is the newest technique for determinationof personal identification and paternity.By using the tech-nology for DNA fingerprints recovered from dry bloodstains,the results of the rese(?)rch of allelefrequency distribution of DNA polymorphism at D2S44and D17S79 loci in 200 Chinese are reported in this pa-per.Based on the frequencies of the various DNA frag-ment sizes,two major allele dusters were detected at theD2S44 locus.One is between 7.7 and 9.2 Kb.with themost common allele at 8.0 Kb,and the other between9.8 Kb and 14.2 Kb and the allele at maximum frequen-cy at 11.1 Kb,in 17.25%.The alleles identified for theD17S79 are found to distribute around the 4 Kb DNA frag-ment sizes and the allele at maximum frequency at 3.4Kb,in 29.55%.Compared with that reported byBalazs I.,there is race difference between Chinese andAmerican blacks and Caucasoids.These results can offerthe numbers for accounting discriminative power and paterri-ty probability in Chinese.