| MacEUSA和IFA对EHF病人血清SIgM的动态检测 |
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| 引用本文： | 刘正稳,张成文,李秀如,岳金声,吴素歌.MacEUSA和IFA对EHF病人血清SIgM的动态检测[J].西安交通大学学报(医学版),1990(3). |
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| 作者姓名： | 刘正稳 张成文 李秀如 岳金声 吴素歌 |
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| 作者单位： | 西安医科大学第一附属医院传染科
(刘正稳，张成文，李秀如，岳金声)，西安医科大学第一附属医院传染科(吴素歌) |
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| 摘 要： | 用IgM抗体捕获法ELISA(MacELISA)和间接免疫荧光法(IFA)同时检测32例流行性出血热(EHF)病人血清特异性lgM抗体(SIgM)的动态反应。二法检出SIgM的总符合率为99.46%,所测抗体滴度的动态反应曲线基本平行,而MacELISA所测抗体滴度比IFA明显提高。早期EHF病人血清即有较高滴度的SIgM抗体,9病日～10病日达高峰,此后趋于下降。IFA法所测不同病日的SIgM滴度无显著差异(F=1.680,P>0.05),而MacELISA则有显著差异(F′=2.978,P<0.05)。二法所测不同病型EHF的SIgM滴度在病日相同时各型间皆无显著差异(P>0.05)。
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| 关 键 词： | 流行性出血热 MacELISA IFA 特异性IgM |
SEQUENTIAL DETERMINATION OF SPECIFIC IGM ANTIBODIES IN SERUM SAMPLES FROM PATIENTS WITH EPIDEMIC HEMORRHAGIC FEVER |
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| Liu Zhengwen,et al.SEQUENTIAL DETERMINATION OF SPECIFIC IGM ANTIBODIES IN SERUM SAMPLES FROM PATIENTS WITH EPIDEMIC HEMORRHAGIC FEVER[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1990(3). |
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| Authors: | Liu Zhengwen |
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| Abstract: | The specific IgM (SIgM) antibodies in serum samples from 32 patients with epidemic hemorrhagic fever (EHF) were sequentially determined by IgM antibody capture ELISA (MacELISA) and indirect immunofluorescence technique (IFA) sitnultaneously. Of the samples detected by the two methods, 99.46% had the same positive or negative results. The response curves of SIgM titres separately determined by MacELISA or IFA were parellel, whereas, the SIgM titres detected by MacELISA were comparatively higher than those detected by IFA. During the course of the disease, no significant defference was found between the SIgM titres of defferent illness types at the same illness day. |
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| Keywords: | epidemic hemorrhagic fever specific IgM antibody IgM antibody capture ELISA indirect immunofluorescence technique |
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