Gαq/11在哇巴因信号转导中的意义

目的观察不同浓度哇巴因对SD大鼠胸主动脉平滑肌细胞Gαq/11表达的影响,并探讨其在哇巴因信号转导中的作用。方法采用贴块法原代培养SD大鼠胸主动脉平滑肌细胞,进行传代和纯化。分别给予生理浓度以及病理浓度的哇巴因干预48 h,免疫细胞化学染色观察平滑肌细胞Gαq/11蛋白的表达。结果生理浓度哇巴因干预后,平滑肌细胞有增殖现象;而病理浓度组细胞发生凋亡。生理浓度组Gαq/11表达(111.21±30.47)显著高于病理浓度组(68.70±6.21)以及对照组(58.81±4.51,P<0.01);病理浓度组和对照组相比无显著变化。结论①Gαq/11介导了生理浓度哇巴因引起细胞增殖的生物学效应;②病理浓度哇巴因引起细胞凋亡的生物学效应与Gαq/11无关;③哇巴因在不同浓度下,可能通过不同的信号转导通路产生相应的药理学作用或生物学效应。

The significance of Gαq/11 protein in ouabain signal transduction

Objective To explore the changes of Gαq/11 expression in smooth muscle cells and its role in ouabain cell signal transduction.Methods The Gαq/11 was analyzed with immunohistochemistry after the primary culture of Sprague-Dawley rats thoracic aorta smooth muscle cells were treated with different concentrations of ouabain for 48 hours.Results The expressions of Gαq/11 protein in the physiological concentration group(111.21±30.47) were significantly higher than those in the pathological concentration group(68.70±6.21) and the control group(58.81±(4.51),P<0.01).There was no significant difference between the pathological concentration group and the control group.Conclusion The proliferation of smooth muscle cells induced by ouabain in physiological concentration is mediated by Gαq/11.The apoptosis of smooth muscle cells induced by ouabain in pathological concentration is not related with Gαq/11.Ouabain might induce different pharmacological or biological effects through different signal transduction pathways.