丹参对恶性梗阻性黄疸治疗作用的实验研究

目的研究丹参对实验性梗阻性黄疸型肝癌发展的影响。方法SD大鼠肝门部肝实质种植Walker-256癌株瘤块,建立恶性梗阻性黄疸模型。将模型鼠分成4组:生理盐水组(n=24)、肌苷+VC组(n=40)、丹参组(n=40)及5-FU组(n=40)。通过腹腔内分别注射生理盐水、肌苷+VC、丹参及5-FU,观察肝功、肝组织形态学及肝癌、癌周、临近肝叶及肺组织中增殖细胞核抗原(PCNA)、血管内皮生长因子(VEGF)及细胞间黏附分子-1(ICAM-1)的表达情况。结果①丹参组对肝功的保护作用明显优于生理盐水组和5-FU组(P<0.01),而与肌苷+VC组相比无显著性差异(P>0.05);同时丹参组对梗阻性黄疸所致的组织形态学损害产生保护作用。②丹参组抑癌率和抑转移率均明显高于生理盐水组和肌苷+VC组(P<0.01);而与5-FU组相比无显著性差异(P>0.05)。③丹参组肝癌、癌周、临近肝组织及肺组织中PCNA、VEGF、ICAM-1的表达均低于肌苷+VC组和生理盐水组(P<0.01);与5-FU组比较,PCNA和VEGF无显著性差异(P>0.05),而ICAM-1则有显著性差异(P<0.05)。结论丹参对恶性梗阻性黄疸的肝脏损害有保护作用,而且通过抑制肝癌细胞增殖、阻碍肿瘤血管的形成及防止肝内外转移等机制而表现出抗癌作用。

Effects of DanShen injection in treating the malignant obstractive jaundice in the SD rat model

Objective To observe the effects of DanShen on the growth of hepatocellular carcinoma in malignant obstructive jaundice in the SD rat model. Methods The model was established by vaccination of transplanting tumour in the porta hepatis with the walker-256 hepatocarcine line, and then obstruction was caused by the infiltration and metastasis of hepatocellular carcinoma. ② The SD rats were divided into four groups: the rats treated by 0.9% NS (n=12), the rats treated by Inosine + Vitamin C (n=20), the rats treated by DanShen (n=20) and the rats treated by 5-FU(n=20). ③ The liver function, morphological changes and the expression of PCNA VEGF and ICAM-1 in carcinoma and peri-carcinoma tissues, adjacent lobe (left-internal lobe) as well as lung tissues were observed after four experimental groups were successively treated by four medicines. ④ Statistical analysis was done to the experimental results. Results ① The growth of rats treated by DanShen injection showed that liver injury caused by malignant obstructive jaundice was significantly relieved compared with liver function and pathological morphological changes in other groups(P<0.01); ② The growth of the groups treaded by DanShen injection, infiltration and metastasis of the hepatocarcinoma and tumor inhibitory rates were decreased compared with that of the groups treated with 0.9%NS and Inosine combined with Vitamin C (P<0.01); and compared with 5-FU group, no significant difference was found(P>0.05); ③ The expression of PCNA VEGF and ICAM-1 in carcinoma, peri-carcinoma adjacent lobe (left-internal lobe) tissues as well as lung tissues were significantly downgraded in groups treated by DanShen injection compared with those in groups treated with 0.9%NS and Inosine combined with Vitamine C (P<0.01); ICAM-1 was also downgraded compared with that in 5-FU group(P<0.05); the expression of PCNA and VEGF in carcinoma, peri-carcinoma and adjacent liver lobe tissue as well as lung tissues showed no difference, between DanShen injection group and 5-FU injection group(P>0.05). Conclusion DanShen injection not only has protective effects on liver injury caused by obstructive jaundice, but the medicine can inhibit the proliferation and growth of hepatocarcinoma, interfere with the vascularization of tumors, and prevent recurrence and metastasis of hepatocarcinoma.