β,β-胡萝卜素改善H_2O_2对成骨细胞损伤的影响

目的研究β,β-胡萝卜素增强成骨细胞抗自由基损伤的作用。方法用H2O2作用M3T3-E1成骨细胞,建立自由基损伤细胞模型。培养的成骨细胞分为对照组、模型组、β,β-胡萝卜素低剂量组(1×10-8mol/L)、β,β-胡萝卜素中剂量组(1×10-7mol/L)和β,β-胡萝卜素高剂量组(1×10-6mol/L)。测定不同处理组细胞活力、超氧化物歧化酶(superoxide dismutase,SOD)活性、活性自由氧(reactive oxygen species,ROS)含量、脂质过氧化物(lipid oxygen,LPO)含量,同时利用荧光偏振法测定细胞膜流动性。结果模型组细胞活力、SOD活性、细胞膜流动性均比不同β,β-胡萝卜素剂量组有显著降低(P<0.01),而ROS、LPO含量均比不同β,β-胡萝卜素剂量组有显著升高(P<0.01)。结论H2O2摄入会导致成骨细胞的氧化损伤,而β,β-胡萝卜素可以预防或降低此类损伤对细胞的影响。

Effect of β,β-carotene in improving H2O2 induced damage of osteoblasts

Objective To investigate the effect of β,β-carotene in enhancing the function of anti-oxidative damage in osteoblasts.Methods M3T3-E1 osteoblasts were randomly divided in five groups: control group,model group,and β,β-carotene group low-dose(1×10-8mol/L),medium-dose(1×10-7mol/L),high-dose(1×10-6mol/L)].The activity of cells,superoxide dismutase(SOD),the content of reactive oxygen species(ROS),lipid oxygen(LPO),and membrane fluidity were tested and compared. Results Compared with those in β,β-carotene groups,the activity of cells,SOD activity and membrane fluidity in the model group were significantly decreased(P<0.01).However,the content of ROS and LPO was significantly raised(P<0.01).Conclusion H2O2 can cause oxidative damage of M3T3-E1 osteoblasts,but β,β-carotene can prevent and decrease its influence.