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| 酪酪肽对胰腺癌细胞生长的抑制作用及Y受体在胰腺癌细胞的表达 | |
| 引用本文： | 李红霞,董蕾,姜炅,王新阳.酪酪肽对胰腺癌细胞生长的抑制作用及Y受体在胰腺癌细胞的表达[J].西安交通大学学报(医学版),2011,32(6):713-716. |
| 作者姓名： | 李红霞 董蕾 姜炅 王新阳 |
| 作者单位： | 1. 西安交通大学医学院第一附属医院消化内科,陕西西安,710061 2. 西安交通大学医学院第二附属医院消化内科,陕西西安,710004 3. 西安交通大学医学院第一附属医院泌尿外科实验室,陕西西安,710061 |
| 摘 要： | 目的 观察酪酪肽(PYY)对体外培养的人胰腺癌Miapaca-2细胞的生长抑制作用及Y受体在胰腺癌细胞的表达.方法 不同浓度的PYY分别作用于体外常规培养Miapaca-2细胞,采用MTT比色法检测细胞增殖情况,光镜观察细胞形态变化.RT-PCR法检测胰腺癌细胞Y受体亚型Y1、Y2、Y5mRNA表达.结果 PYY对Mi... |
| 关 键 词： | 酪酪肽 胰腺癌 Miapaca-2细胞株 Y受体 逆转录-聚合酶链反应 细胞增殖 |
Inhibitory effect of peptide YY and expression of Y receptors on human pancreatic carcinama Miapaca-2 cells |
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| LI Hong-xia,DONG Lei,JIANG Jiong,WANG Xin-yang.Inhibitory effect of peptide YY and expression of Y receptors on human pancreatic carcinama Miapaca-2 cells[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(6):713-716. | |
| Authors: | LI Hong-xia DONG Lei JIANG Jiong WANG Xin-yang |
| Institution: | LI Hong-xia1,DONG Lei2,JIANG Jiong2,WANG Xin-yang3(1.Department of Gastroenterology,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,2.Department of Gastroenterology,the Second Affiliated Hospital,Xi'an 710004,3.Oncological Research Laboratory,China) |
| Abstract: | Objective To observe the effect of peptide YY(PYY) in inhibiting cell growth and detect Y receptors expression of human pancreatic carcinoma Miapaca-2 cell line.Methods The cell line of Miapaca-2 human pancreatic carcinoma was treated with PYY of different concentrations for 24,48 and 72 h,respectively.MTT assay was used to evaluate the cytotoxic effects of PYY on Miapaca-2 cells.Cellular morphous was observed with inverted microscope.The mRNA expressions of Y1,Y2 and Y5 receptor subtypes were detected with... |
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