| 人肝癌细胞HepG2和人正常肝细胞7702的差异蛋白质组学研究 |
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| 引用本文： | 锁爱莉,姚煜,张王刚.人肝癌细胞HepG2和人正常肝细胞7702的差异蛋白质组学研究[J].西安交通大学学报(医学版),2011,32(5):545-549. |
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| 作者姓名： | 锁爱莉 姚煜 张王刚 |
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| 作者单位： | 1. 西安交通大学医学院第一附属医院肿瘤内科,陕西西安,710061
2. 西安交通大学医学院第二附属医院血液内科,陕西西安,710004 |
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| 摘 要： | 目的应用蛋白质组学技术探讨人肝癌细胞HepG2和人正常肝细胞7702的蛋白质差异表达谱,筛选肝细胞癌的特异性标志物。方法应用双向电泳(2-DE)方法对细胞总蛋白进行分离和分析,用质谱(MS)鉴定差异表达蛋白,并用免疫组化方法验证部分差异表达蛋白。结果与7702细胞相比,在HepG2细胞中鉴定出17种差异表达蛋白,10种上调,7种下调,主要涉及物质代谢酶和抗凋亡、调节应激和细胞间信号传导的蛋白质;免疫组化结果验证了膜联蛋白A1(annexin A1)和亲环蛋白A(cyclophilin A)在肝细胞癌病理组织中均明显上调,且主要定位于胞质内。结论人肝癌细胞HepG2和人正常肝细胞7702的蛋白质表达差异显著,其中膜联蛋白A1和亲环蛋白A可作为肝细胞癌诊断及治疗的分子标志物。
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| 关 键 词： | 肝细胞性肝癌 双向电泳 蛋白质组学 免疫组化 膜联蛋白A1 亲环蛋白A |
Comparative proteomics study on HepG2 human hepatocellular carcinoma cells and 7702 human normal hepatocytes |
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| SUO Ai-li,YAO Yu,ZHANG Wang-gang.Comparative proteomics study on HepG2 human hepatocellular carcinoma cells and 7702 human normal hepatocytes[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(5):545-549. |
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| Authors: | SUO Ai-li YAO Yu ZHANG Wang-gang |
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| Institution: | SUO Ai-li1,YAO Yu1,ZHANG Wang-gang2(1.Department of Oncology Medicine,the First Affiliated Hospital,Medical School ofXi'an Jiaotong University,Xi'an 710061,2.Department of Hematology,the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710004,China) |
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| Abstract: | Objective To explore the differential proteomics profiles between HepG2 hepatoma cells and 7702 normal hepatocytes,and to screen specific biomarkers for hepatocellular carcinoma.Methods The cellular total proteins of hepatoma cells and normal hepatocytes were separated using two-dimensional electrophoresis(2-DE).The differentially expressed proteins between the two types of cells were detected and analyzed by imaging analysis,and then the proteins were identified by mass spectrometry.The expression of two i... |
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| Keywords: | hepatocellular carcinoma two-dimensional electrophoresis proteomic approach immunohistochemistry annexin A1 cyclophilin A |
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