| 猪源性CTLA4Ig基因重组腺病毒载体的构建及鉴定 |
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| 引用本文： | 朱海涛,田敏,于良,吕毅,王博.猪源性CTLA4Ig基因重组腺病毒载体的构建及鉴定[J].西安交通大学学报(医学版),2011,32(5):536-539. |
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| 作者姓名： | 朱海涛 田敏 于良 吕毅 王博 |
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| 作者单位： | 西安交通大学医学院第一附属医院肝胆外科,陕西西安,710061 |
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| 摘 要： | 目的 为进行基因转移阻断T细胞共刺激通路诱导异种胰岛细胞移植免疫耐受的研究,构建携带猪源性CTLA4Ig融合基因的重组腺病毒载体.方法 分离猪外周血单个核细胞,经RT-PCR扩增pCTLA4胞外段基因.将PCR产物克隆入pGH IgG,阳性克隆以双酶切及测序鉴定.将目的融合基因pCTLA4Ig克隆人腺病毒穿梭载体pSh...
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| 关 键 词： | pCTLA4基因 腺病毒 载体 基因转移 |
Construction and identification of recombinant adenovirus vector carrying porcine CTLA4Ig |
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| ZHU Hai-tao,TIAN Min,YU Liang,LU Yi,WANG Bo.Construction and identification of recombinant adenovirus vector carrying porcine CTLA4Ig[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(5):536-539. |
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| Authors: | ZHU Hai-tao TIAN Min YU Liang LU Yi WANG Bo |
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| Institution: | ZHU Hai-tao,TIAN Min,YU Liang,Lü Yi,WANG Bo(Department of Hepatobiliary Surgery,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China) |
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| Abstract: | Objective To construct an adenovirus vector containing porcine CTLA4Ig recombinant fusion gene for studying gene transfer to induce immune tolerance of porcine islet xenograft by blockade of T cell co-stimulatory pathway.Methods The extracellular region of the pCTLA4 cDNA was amplified by RT-PCR,and the amplified cDNAs were subcloned into the pGH-IgG plasmid.Positive clones were identified by digesting and sequencing.Adenovirus shuttle plasmid containing pCTLA4Ig recombinant fusion gene was constructed,and ... |
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| Keywords: | pCTLA4 gene adenovirus vector gene transfer |
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