西安汉族、西藏珞巴族DXS6799和HPRTB位点的遗传多态性

目的调查X染色体STR位点DXS6799、HPRTB在西安汉族、西藏珞巴族的遗传多态性。方法应用PCR和聚丙烯酰胺凝胶电泳及银染技术进行基因分型。结果西安汉族DXS6799检出6个等位基因,基因频率在0.0160～0.4840之间,女性和男性的个体识别率分别为0.8516、0.6157;HPRTB位点检出8个等位基因,等位基因频率在0.011～0.290之间,女性和男性的个体识别率分别为0.7974、0.7039。西藏珞巴族DXS6799检出5个等位基因,基因频率为0.0222～0.6074之间,女性和男性的个体识别率分别为0.7684、0.5805;HPRTB位点检出5个等位基因,等位基因频率在0.0420～0.3866之间,女性和男性的个体识别率分别为0.8661、0.7029。西安汉族、西藏珞巴族女性的基因型分布均符合Hardyweinberg平衡。结论DXS6799和HPRTB有较高的个体识别率和非父排除率,在个体识别和亲权鉴定中有应用价值。

Polymorphism of 2 X-STR in Han population in Xi'an and Luoba population in Tibet

Objective To investigate the polymorphism of DXS6799, HPRTB in Xi'an Han population and in Tibet Luoba population. Methods The DXS6799 and HPRTB loci were analyzed by PCR following polyacylamide gel electrophoresis and silver staining. Results Among 123 females and 92 males from Xi'an Han population, 6 alleles were detected in DXS6799 locus, frequencies ranging from 0.016 0 to 0.484 01, PDF: 0.851 6, PDM: 0.615 7 ; while 8 alleles were detected in HPRTB locus, frequencies ranging from 0.011 0 to 0.290 0, PDF: 0.797 4, PDM: 0.703 9. Among 66 females and 28 males from Tibet Luoba population, 5 alleles were detected in DXS6799 locus, frequencies ranging from 0.022 2 to 0.607 4, PDF:0.768 4, PDM: 0.580 5, and 5 alleles were detected in HPRTB locus, frequencies ranging from 0.042 0 to 0.386 6, PDF:0.866 1, PD M :0.702 9. Conclusion The DXS6799 and HPRTB loci are appropriate for individual identification and paternity testing involving a female child.