HSV-1截短gB基因DNA疫苗的构建及初步鉴定 |
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引用本文: | 史霖,范桂香,袁育康,王军阳.HSV-1截短gB基因DNA疫苗的构建及初步鉴定[J].西安交通大学学报(医学版),2001,22(5):425-428. |
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作者姓名: | 史霖 范桂香 袁育康 王军阳 |
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作者单位: | 西安交通大学医学院免疫学教研室, |
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摘 要: | 目的 为有效预防单纯疱疹Ⅰ型病毒 (HSV - 1 )感染 ,给多价DNA疫苗的构建奠定基础 ,构建表达截短HSV - 1 gB糖蛋白的DNA疫苗。方法 用PCR技术从HSV - 1基因组中扩增出编码HSV 1gB糖蛋白 1 - 5 1 7氨基酸序列的一段基因序列 ,通过 pGEM -T中介载体 ,将其插入到真核表达质粒pcDNA 3 1 (+)的CMV启动子下游。结果 构建的重组真核表达质粒可在动物体内正确表达目的基因 ,对HSV - 1病毒攻击具有免疫保护作用。结论 本研究对截短HSV - 1 gB基因DNA疫苗进行了初步的探索性研究 ,也为多价HSV 1DNA疫苗的构建奠定了基础。
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关 键 词: | 单纯疱疹病毒Ⅰ型 糖蛋白B截短基因 DNA疫苗 分子克隆 |
文章编号: | 0258-0659(2001)05-0425-04 |
修稿时间: | 2000年12月16 |
The construction and preliminary appraisement of HSV-1 trunca ted gB gene DNA vaccine |
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Abstract: | Objective In order to prevent infection from herpes simplex virus type 1(HSV-1), and make the found for the construction of multi-valent DNA vaccine, a truncated HSV-1 gB gene DNA vaccine was constructed. Methods By means of PCR technique, a fragment of DNA sequence which encodes the amino acid sequence 1-517 of the HSV-1 glycoprotein B was obtained from the HSV-1 genome. The fragment was then inserted into the lower stream of CMV promoter in the eukaryotic plasmid pcDNA?3.1(+) which had been aided by intermediary vector plasmid pGEMT.Results The recombinant eukaryotic plasmid could correctly express the order gene and induce an immune protection against HSV-1 in vivo .Conclusion This research has paved the way for study on truncated HSV-1 gB gene DNA vaccine, and also make the found for the construction of multi-valent DNA vaccine of HSV-1. |
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Keywords: | herpes simplex virus type Ⅰ glycoprotein B truncated gene DNA vaccine molecular cloning |
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