| ALLELE DISTRIBUTION OF D12S304, D12S313, D12S1583, D12S1640 AND D12S1708 IN CHINESE HAN POPULATION |
| |
| 作者姓名： | 平智广 郭雄 耿冬 康龙丽 左弘 赖江华 |
| |
| 作者单位： | Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China |
| |
| 基金项目： | ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina(No.30371252)andHitechResearchandDevelopmentProgramofChina(863Progam)(No.2002BA711A08) |
| |
| 摘 要： | Analysisofshorttandemrepeat(STR)bypol ymerasechainreaction(PCR)techniqueisare markableimpactindeterminationofbiologicalre latednessbetweenindividualsandinhumanidentity test.DNAtypingafterPCRamplificationisanim portantmethodforlinkageandassociationanalysis…
|
| 关 键 词： | 遗传学 等位基因 中国 汉族人口 多态现象 |
| 文章编号： | 1671-8267（2005）02-0164-04 |
ALLELE DISTRIBUTION OF D12S304, D12S313, D12S1583, D12S1640 AND D12S1708 IN CHINESE HAN POPULATION |
| |
| Ping ZhiGuang;Guo Xiong;Geng Dong;Kang LongLi;Zuo Hong;Lai JiangHua.ALLELE DISTRIBUTION OF D12S304, D12S313, D12S1583, D12S1640 AND D12S1708 IN CHINESE HAN POPULATION[J].Academic Journal of Xi’an Jiaotong University,2005,17(2):164-167. |
| |
| Authors: | Ping ZhiGuang;Guo Xiong;Geng Dong;Kang LongLi;Zuo Hong;Lai JiangHua |
| |
| Institution: | Xi'an Jiaotong University |
| |
| Abstract: | Objective To analyze the genetic polymorphism of 5 STR loci (D12S304, D12S313, D12S1583, D12S1640 and D12S1708) on chromosome 12 in Chinese Han population. Methods EDTA-anticoagulated blood specimens were collected from unrelated individuals of Chinese Han population in Shaanxi province. DNA samples were extracted with the Wizard Genomic DNA purification Kit and were amplified by polymerase chain reaction (PCR) technique. The PCR products were analyzed by ABI 3100 Genetic Analyzer. Results All 5 loci were in Hardy-Weinberg equilibrium. Allele and genotype frequencies, heterozygosity, power of discrimination, polymorphism information content, probability of paternity exclusion and matching probability of each locus were calculated in Excel 2002. Conclusion They are complex loci with lots of evenly distributed alleles and high heterozygosity in Chinese Han population. Thus they are informative polymorphic loci and valuable DNA marker which represents a superior alternative to many established STRs. |
| |
| Keywords: | short tandem repeat (STR) genetic polymorphism Hardy-Weinberg equilibrium chromosome 12 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
