| 流式细胞术CD45/SSC设门在急性白血病免疫分型中的应用 |
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| 引用本文： | 张玮,程小丽,杨玉琮,陈葳,王翔,周萍,尤元义. 流式细胞术CD45/SSC设门在急性白血病免疫分型中的应用[J]. 西安交通大学学报(医学版), 2007, 28(4): 462-463,F0004 |
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| 作者姓名： | 张玮 程小丽 杨玉琮 陈葳 王翔 周萍 尤元义 |
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| 作者单位： | 1. 西安交通大学,理学院,陕西西安,710061
2. 西安交通大学,医学院第一附属医院实验医学中心,陕西西安,710061 |
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| 摘 要： | 目的探讨流式细胞术CD45／SSC设门在急性白血病免疫分型中的应用。方法选用三色荧光抗体直接标记初诊急性白血病患者骨髓，应用流式细胞术CD45／SSC设门进行免疫分型。结果49例急性白血病患者分为三型，其中系列专一型25例，占51．2％；系列非专一型21例，占42．8％；裸型3例，占6．0％。结论流式细胞术CD45／SSC设门，容易将原始／幼稚细胞与正常成熟细胞群区分开，使白血病分型结果更准确、可靠。
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| 关 键 词： | 流式细胞术 急性白血病 免疫分型 |
| 文章编号： | 1671-8259（2007）04-0462-02 |
| 修稿时间： | 2006-05-082006-12-05 |
Application of CD45/SSC gating in acute leukemia immunophenotyping |
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| Zhang Wei,Cheng Xiaoli,Yang Yucong,Chen Wei,Wang Xiang,Zhou Ping,You Yuanyi. Application of CD45/SSC gating in acute leukemia immunophenotyping[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2007, 28(4): 462-463,F0004 |
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| Authors: | Zhang Wei Cheng Xiaoli Yang Yucong Chen Wei Wang Xiang Zhou Ping You Yuanyi |
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| Abstract: | Objective To investigate the application of CD45/SSC gating in acute leukemia immunophenotype.Methods Three-color monoclonal antibodies were used to mark the marrows of acute leukemia patients,and CD45/SSC gating in flow cytometry was used for immunofluorescence.Results The 49 cases of acute leukemia immunophenotype were classified into 3 subgroups: lineage specific antigen expression(25 cases),aberration antigen expression(21 cases),and null type(3 cases).The percentage of each subgroup was 51.2%,42.8% and 6.0%,respectively.Conclusion CD45/SSC gating technique can easily discriminate mature leukemia cells from normal cells,thus rendering more reliable and precise immunophenotype results. |
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| Keywords: | CD45 |
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