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CD_3 McAb联合rhIL-2激活的骨髓对白血病细胞杀伤净化作用的研究
引用本文:魏绪仓,邢佩霓,赵文理,杨娣娣,韩秀蕊,李梅生. CD_3 McAb联合rhIL-2激活的骨髓对白血病细胞杀伤净化作用的研究[J]. 西安交通大学学报(医学版), 2002, 23(6): 582-584,612
作者姓名:魏绪仓  邢佩霓  赵文理  杨娣娣  韩秀蕊  李梅生
作者单位:陕西省人民医院血液病研究室,西安,710068
基金项目:陕西省卫生厅中青年科研基金资助 (No .98SM 71)
摘    要:目的 探讨CD3单克隆抗体 (CD3McAb)联合重组人白细胞介素 2 (rhIL 2 )激活的骨髓对白血病细胞的杀伤及净化作用。方法 用MTT法检测激活骨髓的细胞毒作用 ;半固体集落培养法观察激活骨髓CFU GM水平和对白血病细胞的净化作用 ;采用间接荧光法测定激活骨髓的免疫标记。结果 CD3McAb与rhIL 2联合激活的骨髓对白血病细胞株K5 6 2、HL 6 0均有明显的杀伤净化作用 ,且优于rhIL 2或CD3McAb单用组 (P <0 .0 5或P <0 .0 1)。rhIL 2和 (或 )CD3McAb对激活骨髓的CFU GM水平无明显影响。CD3McAb +rhIL 2激活的骨髓 ,与活化前及rhIL 2或CD3McAb组相比 ,CD3+ 、CD8+ 、CD1 9+ 、CD2 5+ 、CD38+ 、CD56+ 细胞显著升高。结论 CD3McAb能增强rhIL 2激活的骨髓对白血病细胞的杀伤及净化作用

关 键 词:CD3McAb  rhIL2  激活骨髓  杀伤活性  骨髓净化
文章编号:1671-8259(2002)06-0582-03

Study of bone marrow activated with CD3 monoclonal antibody and rhinterleukin-2 for killing and purging leukemia cells in vitro
Wei Xucang,Xing Peini,Zhao Wenli,Yang Didi,Han Xiurui,Li Meisheng. Study of bone marrow activated with CD3 monoclonal antibody and rhinterleukin-2 for killing and purging leukemia cells in vitro[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2002, 23(6): 582-584,612
Authors:Wei Xucang  Xing Peini  Zhao Wenli  Yang Didi  Han Xiurui  Li Meisheng
Abstract:Objective To study the role of bone marrow activated in vitro with CD 3 monoclonal antibody(CD 3McAb) and rhinterleukin 2(rhIL 2)for killing and purging leukemia cells. Methods The cytotoxicity of activating bone marrow(ABM) was measured by using methyl thiazolyl tetraolium(MTT) assay. The numbers of CFU GM and action of purging of ABM were counted by semi solid culture of colonies. The phenotypes of ABM were analysed by using indirect immunofluorescence assay. Results The significant roles of killing and purging K562 cells and HL 60 cells through bone marrow activated CD 3McAb group( P <0.05 or P <0.01). The levels of CFU GM of ABM had no significant effect when using CD 3McAb or rhIL 2. The expressions of CD3, CD8, CD19, CD25, CD38 and CD56 on the surface of ABM with CD 3McAb and rhIL 2 were significantly promoted when compared to pre culture of any single group. Conclusion The action of killing and purging leukemia cells by using bone marrow activated with rhIL 2 can be strengthened when combined with CD 3McAb. This study may provide a theoretical and experimental basis for auto BMT's action of purging in vitro .
Keywords:CD 3monoclonal antibody  rhinterleukin 2  activated bone marrow  killing activity  bone marrow purging
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