CD_3 McAb联合rhIL-2激活的骨髓对白血病细胞杀伤净化作用的研究

目的　探讨CD3单克隆抗体 (CD3McAb)联合重组人白细胞介素 2 (rhIL 2 )激活的骨髓对白血病细胞的杀伤及净化作用。方法　用MTT法检测激活骨髓的细胞毒作用 ;半固体集落培养法观察激活骨髓CFU GM水平和对白血病细胞的净化作用 ;采用间接荧光法测定激活骨髓的免疫标记。结果　CD3McAb与rhIL 2联合激活的骨髓对白血病细胞株K5 6 2、HL 6 0均有明显的杀伤净化作用 ,且优于rhIL 2或CD3McAb单用组 (P <0 .0 5或P <0 .0 1)。rhIL 2和 (或 )CD3McAb对激活骨髓的CFU GM水平无明显影响。CD3McAb +rhIL 2激活的骨髓 ,与活化前及rhIL 2或CD3McAb组相比 ,CD3+ 、CD8+ 、CD1 9+ 、CD2 5+ 、CD38+ 、CD56+ 细胞显著升高。结论　CD3McAb能增强rhIL 2激活的骨髓对白血病细胞的杀伤及净化作用

Study of bone marrow activated with CD3 monoclonal antibody and rhinterleukin-2 for killing and purging leukemia cells in vitro

Objective To study the role of bone marrow activated in vitro with CD 3 monoclonal antibody(CD 3McAb) and rhinterleukin 2(rhIL 2)for killing and purging leukemia cells. Methods The cytotoxicity of activating bone marrow(ABM) was measured by using methyl thiazolyl tetraolium(MTT) assay. The numbers of CFU GM and action of purging of ABM were counted by semi solid culture of colonies. The phenotypes of ABM were analysed by using indirect immunofluorescence assay. Results The significant roles of killing and purging K562 cells and HL 60 cells through bone marrow activated CD 3McAb group( P <0.05 or P <0.01). The levels of CFU GM of ABM had no significant effect when using CD 3McAb or rhIL 2. The expressions of CD3, CD8, CD19, CD25, CD38 and CD56 on the surface of ABM with CD 3McAb and rhIL 2 were significantly promoted when compared to pre culture of any single group. Conclusion The action of killing and purging leukemia cells by using bone marrow activated with rhIL 2 can be strengthened when combined with CD 3McAb. This study may provide a theoretical and experimental basis for auto BMT's action of purging in vitro .