| Flavopiridol诱导多发性骨髓瘤细胞凋亡的分子机制 |
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| 引用本文： | 王孟昌,张斌. Flavopiridol诱导多发性骨髓瘤细胞凋亡的分子机制[J]. 西安交通大学学报(医学版), 2011, 32(2): 201-204 |
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| 作者姓名： | 王孟昌 张斌 |
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| 作者单位： | 1. 西安交通大学医学院第一附属医院血液科,陕西西安,710061
2. 美国Maryland癌症中心,Baltimore 21205,USA |
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| 基金项目： | 国家自然科学基金资助项目(No.81071952)~~ |
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| 摘 要： | 目的探讨flavopiridol诱导多发性骨髓瘤(MM)细胞凋亡的分子机制。方法以TUNEL法检测细胞凋亡,Western blot测定Mcl-1、CyclinD2、Bcl-XL、Bag-1和XIAP等。结果在临床剂量的flavopiridol浓度下,MM细胞系在加药后3 h内出现快速凋亡。不同剂量的flavopiridol均可快速诱导Mcl-1表达下调和CTD磷酸化的抑制,flavopiridol抑制CTD磷酸化的剂量与其抑制Mcl-1表达的剂量密切相关。该剂量也是其诱导MM细胞凋亡的剂量。转录抑制因子放线菌素D及flavopiridol均能抑制Mcl-1表达,并可诱导原代MM细胞凋亡。结论转录抑制因子放线菌素D和CDK抑制剂flavopiridol诱导MM细胞凋亡可能与其抑制CTD磷酸化,从而抑制Mcl-1的mRNA以及蛋白质水平有关。
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| 关 键 词： | 多发性骨髓瘤 flavopiridol Mcl-1 Bcl-XL Bag-1 CyclinD2 |
Molecular mechanisms of flavopiridol in inducing apoptosis in multiple myeloma |
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| WANG Meng-chang,ZHANG Bin. Molecular mechanisms of flavopiridol in inducing apoptosis in multiple myeloma[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2011, 32(2): 201-204 |
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| Authors: | WANG Meng-chang ZHANG Bin |
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| Affiliation: | WANG Meng-chang1,ZHANG Bin2(1.Department of Hematology,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China,2.University of Maryland Cancer Center,Baltimore 21205,USA) |
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| Abstract: | Objective To study the molecular mechanisms of flavopiridol in inducing apoptosis in multiple myeloma(MM).Methods TUNEL methods were used to detect cell apoptosis;Western blot was used to determine the expression levels of myeloid cell leukemia-1(Mcl-1),CyclinD2,Bcl-XL,Bag-1 and XIAP.Results The clinical concentration of flavopiridol resulted in rapid apoptosis after dosing within 3h in MM cell lines.Different doses of flavopiridol could induce rapid down-expression of Mcl-1 and inhibition of CTD phosphoryl... |
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| Keywords: | multiple myeloma flavopiridol Mcl-1 Bcl-XL Bag-1 CyclinD2 |
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