THE EXPRESSION OF BCL-2 GENE AFTER TRANSIENT FOCAL ISCHAEMIA AND THE EFFECT OF MK-801

Thesequenceofeventsthroughwhichischaemialeadstocelldeathispoorlyunderstood.Anumberofendogenouscomponentshavebeenimplicatedinthesubsequentneurodegenerativeprocess,suchasglutamate,freeradicals,calciumandpolyamines,allofwhichhavebeenshowedtobeelevatedinassociationwithischaemicinsults.Agentssuchasglutamineandcalciumhaveestablishedrapidandpotentphysiologicalactions,thatareexaggeratedduringischaemia,leadingtoamassiveanduncontrolledamplificationoftheirnormaleffects.Animportantcomponentofthephysiologi…

THE EXPRESSION OF BCL-2 GENE AFTER TRANSIENT FOCAL ISCHAEMIA AND THE EFFECT OF MK-801

in order to clarify the pesible role of the bcl-2 gene imolved in the cell death Program,and the relatiouship of glutamate receptors with bcl-2 gene expressin, this study examied the expression of bcl-2 gene protein, the neuronal status of apoptosis and the effects of MK-801 using immunohistochemistry and in situ terminal.labelling methods after 30 min of.middle cerebral artery(MCA) occlusion and followed by 24 h of reperfusion. The presence of bcl-2 gene protein increased in the ipeilateral hemisphere of ischaemis espeially in the MCA territory MK-801 enhanced the expresion of the bcl-2 gene protein. No DNA fragmentation was detected in this experiment. In conclusion. bcl-2 gene activity increased during transient focal ischaemia, and was potentiated by MK MK801, which may be an endogenous protective mechanism .against ischaemic apoptosis. Apoptosis wasnot detected after tranient focal ischoemia. for 30 min rollowed by 24 h of reperfusiou.