| 人胎胰组织培养后的B细胞功能测定及电镜观察 |
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| 引用本文： | 寒梅,高慧,王惠芳,杨少毅,徐利,新华.人胎胰组织培养后的B细胞功能测定及电镜观察[J].西安交通大学学报(医学版),1989(3). |
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| 作者姓名： | 寒梅 高慧 王惠芳 杨少毅 徐利 新华 |
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| 作者单位： | 西安医科大学内分泌代谢病研究室
(寒梅，高慧，王惠芳，徐利)，西安医科大学电子显微镜室
(杨少毅)，西安医科大学内分泌代谢病研究室(新华) |
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| 摘 要： | 本文报道了经Ham's F_(10)培养的人胎胰组织胰岛细胞功能状况及扫描电镜检查所见.发现培养物的胰岛素和C 肽分泌功能从被培养后的第3天开始增强,第7天后减弱,第10天后明显低落,分泌曲线呈抛物线型。在其分泌功能旺盛的3～7天期间,电子显微镜下可见群集的胰岛B 细胞颗粒占总体的3/4,也可见散在的胰岛A 细胞.7天以后的功能衰退期,则见A、B 细胞分泌颗粒电子密度减低,影像模糊,难以辨认.
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| 关 键 词： | 人胎胰组织 胰岛细胞 胰岛素 C-肽 |
MEASUREMENT AND ELECTROMICROSCOPIC OBSERVATION OF HUMAN FETAL PANCREATIC TISSUES AFTER CULTURE |
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| Han Mei,et al.MEASUREMENT AND ELECTROMICROSCOPIC OBSERVATION OF HUMAN FETAL PANCREATIC TISSUES AFTER CULTURE[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1989(3). |
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| Authors: | Han Mei |
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| Institution: | Research Laboratory of Endocrinology and Metabolism Diseases |
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| Abstract: | This artical reports pancreatic islet cells function and electromicroscopic observation of human fetal pancreatic tissues cultured by Han's F10.It is foundthat the secretic function of insulin and C-peptide inthe cultrued cells gradually increased the third day afterbeing cultured,decreased after the seven day,and de-clined significantly after the tenth day.The secreticcurve was of a parabolic type.During the hypersecreticperiod from the third day to the seventh day after cul-ture,clustered B islet cells were found under theelectromicroscopic,accounting for 3/4 of the total andthe sporadic type A islet cells.The seventh day after cul-ture,the electronic density of the secretic particles inthe type A and type B islet cells declined and becameblurred and unable to be seen clearly. |
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| Keywords: | human fetal pancreatic tissues B islet cells insulin C-peptide |
| 本文献已被 CNKI 等数据库收录！ |
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