基因修饰小鼠应用于心脏研究的进展:从小鼠到人类,从基因到临床

基因修饰(敲除、变异和过度表达)小鼠自90年代初应用于心脏学研究。近十余年来,利用这一类模型的研究发现对心血管领域所取得的重要进展有着突出的贡献,已经成为该领域里具有举足轻重地位的研究手段。目前,基因修饰小鼠模型的数量日益增多并已形成系统,有关的心脏表现型数据库日趋丰富,多种从整体到分子水平的表现型的研究手段已十分成熟。在今后的心脏研究中,小鼠模型有望对阐明疾病的分子机制和探索新的治疗途径起到很大的推动作用。

From mouse to man, from gene to clinic: Progress in heart research using genetically modified mouse models

Since the 1990s,models of genetically modified mice(gene knockout,mutation or transgene overexpression) have been increasingly used in heart research.The findings from this class of murine models have contributed significantly to a number of research breakthroughs achieved in the last 15 years.Currently,the number of genetically modified mouse strains has been increasing steadily.These models that targeted a family or a functionally relevant group of proteins have formed systemic tools for research use.The database of mouse cardiac phenotype has been established.Meanwhile,the research methodology and strategic approach exploring mouse cardiac phentypes at molecular to in vivo levels have been mature.It is expected that the use of this class of genetically targeted mouse models will become increasingly important in future research.