| 谷胱甘肽对Cu~(2+)-H_2O_2导致关节软骨蛋白聚糖降解的影响 |
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| 引用本文： | 俞小瑞,田梦玉. 谷胱甘肽对Cu~(2+)-H_2O_2导致关节软骨蛋白聚糖降解的影响[J]. 西安交通大学学报(医学版), 1995, 0(3) |
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| 作者姓名： | 俞小瑞 田梦玉 |
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| 作者单位： | 西安医科大学生物化学教研室 西安710061 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 应用小牛关节软骨短期器官温育的方法,研究了谷胱甘肽(GSH)对Cu~(2+)-H_2O_2导致关节软骨蛋白聚糖(PG)降解的影响。结果表明:GSH能非常显著地抑制Cu(2+)-H_2O_2对软骨PG的降解作用,并呈剂量依赖型。用Sepharosc 6B柱层析分析软骨PG降解产物,发现GSH还能消除Cu~(2+)-H_2O_2导致的PG链的断裂,保护PG结构的完整。GSH对Cu~(2+)-H_2O_2的作用机理可能一方面与Cu~(2+)形成了复合物,另一方面使H_2O_2还原为H_2O,而消除了两者对软骨PG的降解作用。
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| 关 键 词： | 谷胱甘肽 Cu~(2+)-H_2O_2 关节软骨 蛋白聚糖 |
EFFECT OF GLUTATHIONE ON DEGRADATION OF PROTEOGLYCAN OF ARTICULAR CARTILAGE TREATED BY Cu~(2 )-H_2O_2 |
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| Yu Xiaorui,Tian Mengyu. EFFECT OF GLUTATHIONE ON DEGRADATION OF PROTEOGLYCAN OF ARTICULAR CARTILAGE TREATED BY Cu~(2 )-H_2O_2[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 1995, 0(3) |
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| Authors: | Yu Xiaorui Tian Mengyu |
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| Affiliation: | Department of Biochemistry |
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| Abstract: | The effect of glutathione (GSH) on degradation of proteoglycan (PG) of articular cartilage treated by Cu2 -H2O2 was studied with incubating articular cartilage slices of new born calf. The result indicated that GSH could inhibit the degradation of PG mediated by Cu2 -H2O2 very significantly, and showed in a dosage-dependant manner. Analysis of the degradation products of PG on Sepharose 6B chromatography showed that GSH wasable to prevent the breakage of PG molecular led by Cu2 -H2O2. to protect cartilage PG structure from destruction. The mechanism of GSH inhibition on the degradation of PG mediated by Cu2 -H2O2 may be that GSH can not only be combined with Cu2 to form a complex but also reduce H2O2 to H2O to antagonize the action of Cu2 -H2O2 on the degradation of PG. |
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| Keywords: | glutathione Cu2 -H2O2 articular cartilage proteoglycan |
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