卡托普利对系膜增殖型肾炎家兔系膜基质的影响

目的　探讨血管紧张素转换酶抑制剂卡托普利对系膜增殖型肾炎家兔系膜细胞增殖、系膜基质增多的影响及其机制。方法　将雄性日本大耳白兔 2 4只随机分成正常对照组、病理模型对照组和卡托普利治疗组。建立家兔系膜增殖型肾炎模型。双缩脲法测定卡托普利对系膜增殖型肾炎家兔 2 4h尿蛋白排泄量的影响 ;放免法测定血浆中内皮素 (ET 1)及降钙素基因相关肽 (CGRP)的水平 ;自动图像分析仪测定各组肾小球的定量指数、系膜基质指数 ;用免疫组化法观察肾组织增殖性细胞核抗原 (PCNA)表达。结果　与病理模型对照组相比 ,卡托普利治疗组家兔 2 4h尿蛋白排泄量明显下降 (P <0 .0 5 ) ,系膜面积显著减少 (P <0 .0 5 ) ,ET 1降低、CGRP升高 (P <0 .0 5 ) ,PCNA阳性细胞数减少 (P <0 .0 5 )。结论　卡托普利通过调节内皮素与降钙素基因相关肽病理性改变 ,减少系膜细胞的增殖及系膜基质的增多 ,对系膜增殖型肾炎家兔起到保护作用。

Effect of captopril on mesangial matrix of mesangial proliferative glomerulonephritis in rabbits

Objective To investigate the ef fe ct of captopril, angiotensin converting enzyme inhibitor (ACEI), on the clinical histological changes of mesangial proliferative glomerulonephritis (MSPGN) in r abbits. Methods A total of 24 male rabbits were randomly divided in to 3 groups: normal control group (N group), untreated group (M group), and capt opril-treated group (C group). The rabbit MSPGN models were established. 24 hours' excretion quantity of urine protein (E QUP) was determined every week, and the levels of plasma endothelin-1 (ET-1) a nd calcitonin gene-related peptide (CGRP) were tested with radioimmunoassay (RI A), mesangial matrix expansion were evaluated with quantitative pathologic analy sis. Immunohistochemical staining for proliferating cell nuclear antigen (PCNA) was performed after treatment. Results EQUR mesangial matrix expansion ET-1 in C group wa s significantly lower than that in M group (P< 0.05) while CGRP was remar kably higher (P< 0.05). The number of PCNA was remarkably smaller (P< 0.05). Damage of renal tissue in C group was milder compared with that in M group. Conclusion Captopril can decrease mesangial matr ix expansion, alleviate the injury of renal tissue and achieve markedly therapeu tic effect.