产前束缚应激刺激对子代大鼠脑海马中Bax、Bcl-2、Fas和FasL表达的影响

目的　确定产前束缚应激刺激对子代大鼠脑海马中Bax、Bcl-2、Fas和FasL表达的影响。方法　在母鼠怀孕的13~19 d,每天进行3次束缚应激,每次45 min。于雌、雄仔鼠1 月龄时对脑组织切片应用免疫组化方法检测海马中Bax、Bcl-2、Fas 和 FasL的表达。结果　应激组 Bax的表达强于对照组(P<0.05), Bcl -2 的表达弱于对照组(P<0.05),Fas和FasL的表达两组间没有差异(P>0.05)。结论　产前束缚应激刺激可使子代大鼠脑海马神经元中 Bax表达上调,Bcl-2表达下调,对Fas和FasL的表达没有影响。

Effect of prenatal restraint stress on the expressions of Bax, Bcl-2, Fas and FasL in hippocampus of the offspring

Objective To study the effect of chronic restraint stress on the expressions of Bax, Bcl-2, Fas and FasL in hippocampus of the offsprings during the pregnancy. Methods Restraint stress was performed three times each day of pregnant women from day 13 to day 19 (each time for 45min). After delivery, the expression of Bax, Bcl-2, Fas and FasL in hippocampus of one-month-old female and male rats were respectively examined by immunohistochemical method. Results Compared with the control group, the expression level of Bax in stressed group is higher (P<0.05), and the expression level of Bcl-2 is lower (P<0.05); there is no significant difference in the expression of Fas and FasL(P> 0.05). Conclusion Restraint stress during the pregnancy can upgrade the expression of Bax and degrade the expression of Bcl-2 of the offsprings; no effect was found on the expressions of Fas and FasL of the offsprings.