| EP_2在海马齿状回突触传递中的作用 |
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| 引用本文： | 杨红卫.EP_2在海马齿状回突触传递中的作用[J].西安交通大学学报(医学版),2010,31(1). |
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| 作者姓名： | 杨红卫 |
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| 作者单位： | 三峡大学医学院生理学教研室,湖北宜昌,443002 |
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| 基金项目： | 湖北省卫生厅科研基金，湖北省教育厅资助项目 |
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| 摘 要： | 目的 探讨EP_2在小鼠海马齿状回突触传递中的作用及其机制.方法 细胞外记录技术记录海马脑片穿通纤维-齿状回颗粒细胞突触的场兴奋性突触后电位(fEPSP).结果 ①EP_2的激动剂Butaprost增强海马齿状回突触传递和降低双脉冲比(paired-pulse ratio, PPR),此效应被EP_2的拮抗剂AH6809所阻断.②Forskolin单独或Forskolin+AH6809均可增强fEPSP的斜率.③EP_2增强突触效能的效应由PKA,ERK和IP_3途径介导.结论 多信号转导途径可能参与EP_2激活诱导的突触传递增强.
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| 关 键 词： | 前列腺素 环氧合酶-2(COX-2) 突触传递 海马 |
Role of EP_2 in synaptic transmission in dentate gyrus of hippocampus |
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| YANG Hong-wei.Role of EP_2 in synaptic transmission in dentate gyrus of hippocampus[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(1). |
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| Authors: | YANG Hong-wei |
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| Institution: | YANG Hong-wei(Department of Physiology,College of Medical Science ofThree Gorges University,Yichang 443002,China) |
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| Abstract: | Objective To evaluate the role of EP_2 in synaptic transmission in dentate gyrus of hippocampus. Methods Field excitatory post-synaptic potentials (fEPSP) were recorded at the perforated path-granule neurons in dentate gyrus in vitro. Results ① Butaprost, an agonist of EP_2, enhanced the synaptic transmission in dentate gyrus and decreased the paired-pulse ratio, and these effects were reversed by bath application of AH6809 (EP_2 antagonist). ② Application of Forskolin alone or with AH6809 elevated the slope of fEPSP. ③ The Butaprost-induced responses were mediated via PKA, ERK and IP_3 signal pathways. Conclusion Multiple signal pathways were involved in the EP_2 activation-mediated enhancement of synaptic transmission. |
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| Keywords: | EP_2 prostaglandin cyclooxygenase-2 (COX-2) EP_2 synaptic transmission hippocampus |
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