|
|||||
|
|
| 非洛地平对ox-LDL损伤的人脐静脉内皮细胞一氧化氮合酶mRNA及一氧化氮表达的影响 | |
| 引用本文： | 盛婴,祁杰,郑见宝,袁祖贻.非洛地平对ox-LDL损伤的人脐静脉内皮细胞一氧化氮合酶mRNA及一氧化氮表达的影响[J].西安交通大学学报(医学版),2010,31(4). |
| 作者姓名： | 盛婴 祁杰 郑见宝 袁祖贻 |
| 作者单位： | 1. 西安交通大学医学院第一附属医院,干部病房,陕西西安,710061 2. 西安交通大学医学院第一附属医院,心血管内科,陕西西安,710061 3. 西安交通大学医学院第一附属医院,普通外科,陕西西安,710061 |
| 摘 要： | 目的 探讨非洛地平对氧化型低密度脂蛋白(ox-LDL)损伤的人脐静脉内皮细胞(HUVEC)内皮型一氧化氮合酶(eNOS)、诱生型一氧化氮合酶(iNOS)mRNA及一氧化氮(NO)表达的影响.方法 将原代细胞分为空白对照组、ox-LDL(6、12.5、25mg/L)损伤组、非洛地平(0.1、1.0、10μmol/L)+ox-LDL(25mg/L)干预组,采用实时荧光定量PCR技术检测eNOS、iNOS的mRNA水平,硝酸还原酶法测定培养上清中NO的浓度.结果 ox-LDL损伤内皮细胞后,eNOS、iNOS mRNA及NO较空白对照组升高;非洛地平下调ox-LDL损伤的内皮细胞iNOS的表达,增加其NO的生成.结论 非洛地平可抑制低浓度ox-LDL诱导的iNOS mRNA表达,增加NO的生成,发挥其保护内皮细胞的功能. |
| 关 键 词： | 非洛地平 一氧化氮合酶 一氧化氮 动脉粥样硬化 |
Effects of felodipine on the secretion of nitricoxide synthase and nitrogen monoxidum from human endothelial cells injuryed by oxidized low density lipoprotein |
|
| SHENG Ying,QI Jie,ZHENG Jian-bao,YUAN Zu-yi.Effects of felodipine on the secretion of nitricoxide synthase and nitrogen monoxidum from human endothelial cells injuryed by oxidized low density lipoprotein[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(4). | |
| Authors: | SHENG Ying QI Jie ZHENG Jian-bao YUAN Zu-yi |
| Institution: | SHENG Ying1,QI Jie2,ZHENG Jian-bao3,YUAN Zu-yi2(1. Department of Cadres' Wards,2. Department of Cardiovasology,3. Department ofGeneral Surgery,the First Affiliated Hospital,Medical School ofXi'an Jiaotong University,Xi'an 710061,China) |
| Abstract: | Objective To investigate the protective effects of felodipine on mRNA levels of endothelial nitricoxide synthase (eNOS) and inducible nitricoxide synthase (iNOS) as well as the level of nitrogen monoxidum (NO) from human umbilical vein endothelial cells (HUVECs) injuryed by oxidized low density lipoprotein (ox-LDL). Methods Isolated HUVECs were divided into blank control group,ox-LDL injury group treated with ox-LDL of different concentrations (6,12.5 and 25 mg/L),and intervention group of felodipine (0.1,1... |
| Keywords: | felodipine nitricoxide synthase nitrogen monoxidum atherosclerosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |