参芪注射液对H22荷瘤小鼠抑瘤作用的细胞免疫机制

目的研究参芪注射液的体内抑瘤效应与荷瘤机体细胞免疫功能的关系,探讨其抑瘤作用的细胞免疫学机制。方法建立小鼠H22肝癌实体瘤模型,分别给予高、中、低剂量参芪注射液,测定各组小鼠肿瘤生长抑制率(IR)和腹腔巨噬细胞的吞噬指数;采用MTT法测定各组小鼠脾淋巴细胞增殖指数;ELISA法检测各组小鼠血清中白细胞介素-2(IL-2)和γ-干扰素(IFN-γ)水平。结果高、中剂量参芪注射液实验组小鼠H22实体瘤IR分别为60.72%和48.65%,与模型组比较有显著性差异(P<0.05);参芪注射液实验组小鼠脾脏淋巴细胞增值指数、腹腔巨嗜细胞吞噬指数、血清IL-2和IFN-γ的含量都显著高于模型组小鼠(P<0.05)。结论参芪注射液通过提高荷瘤小鼠的细胞免疫功能,改善荷瘤小鼠的抗肿瘤免疫状态,协同对肿瘤细胞的直接杀伤作用,在荷瘤小鼠体内发挥其抑瘤抗瘤作用。

Cellular immune mechanism of anti-tumor efficacy of Shenqi injection in H22 tumor-bearing mice

Objective To investigate the relationship between in vivo anti-tumor efficacy of Shenqi injection and its effect on immunologic function in H22 tumor-bearing model mice,and explore the cellular immunologic mechanism of Shenqi injection in inhibiting tumor growth.Methods Three different doses of Shenqi injection were given to H22 tumor-bearing model mice in treatment groups.The tumor growth inhibitory rate(IR) and the index of phagocytosis of peritoneal macrophage were calculated.MTT assay was used to observe the effect of Shenqi injection on spleen lymphocytes stimulated by ConA in vitro separated from H22 tumor-bearing mice.Murine serum IL-2 and IFN-γ were detected by means of ELISA assay.Results IR was significantly reduced in two treatment groups compared with that of the model mice(60.72%,48.65%,P<0.05).The spleen lymphocyte stimulation index,the index of phagocytosis,and the level of serum IL-2 and IFN-γ were significantly increased in treatment groups compared with those of the model groups(P<0.05).Conclusion Shenqi injection may directly kill the tumor cells by increasing the cellular immunologic function or improving the anti-tumor immunologic function in tumor-bearing mice.