中药宝宝乐对幼龄厌食大鼠脑肠肽的调节作用

目的研究运脾平肝复方宝宝乐口服液对小儿厌食症动物模型胃泌素、β-内啡肽(-βEP)及胆囊收缩素(CCK)的调节作用。方法病因模拟法制作幼龄厌食大鼠模型,用宝宝乐治疗,放射免疫检测技术测定下丘脑、胃窦部及外周血胃泌素、-βEP和CCK的含量,与单纯运脾复方儿宝颗粒对照。结果模型组胃窦部胃泌素减少(P<0.05)、血清水平无明显变化;胃窦部及血浆中-βEP下降(P<0.01,P<0.05),下丘脑无明显变化;下丘脑和血浆CCK-8均增高(P<0.01);治疗组胃窦部胃泌素含量、胃窦部及血浆-βEP、下丘脑和血浆CCK-8均恢复正常,宝宝乐组下丘脑CCK-8回降较儿宝组明显(P<0.05)。结论宝宝乐能调节该模型中枢及外周胃泌素、-βEP和CCK的分泌与释放。

Regulative action of Bao Bao Le Liquid on brain-gut peptide in juvenile rat model of anorexia

Objective To study the regulative action of Bao Bao Le Liquid on central and peripheral gastrin,β-endorphin(β-EP) and cholocystokinin-octapeptide(CCK-8) in juvenile rat model of anorexia.Methods Juvenile rat model of anorexia was established by imitating the major cause of infantile anorexia and treated with Bao Bao Le Liquid.The gastrin β-EP and CCK-8 concentration in hypothalamus,antrum pyloricum and peripheral blood were examined by radioimmunoassay.Results The gastrin level of model rats decreased in gastric antrum(P<0.05),but no change was observed in plasma.The β-EP level in gastric antrum and plasma decreased(P<0.05),but no change was observed in hypothalamus.The CCK-8 level in hypothalamus and plasma showed a significant increase((P<0.05) and P<0.01).After treatment with Bao Bao Le Liquid and Er Bao Granules,the gastrin level in gastric antrum,the β-EP level in gastric antrum and plasma,and the CCK-8 level in hypothalamus and plasma were normal but the Bao Bao Le group showed a more significant decrease in hypothalamic CCK-8 level than that of Er Bao group(P<(0.05)).Conclusion Bao Bao Le Liquid can regulate the secretion and release of gastrin,β-EP and CCK in central and peripheral areas and can play an important role in inhibiting the secretion and release of CCK in our model hypothalamus.