Biological effects of Mississippi River nitrogen on the northern gulf of Mexico—a review and synthesis

The Mississippi River currently delivers approximately 1.82 Tg N year⁻¹ (1.3×10¹¹ mol N year⁻¹) to the northern Gulf of Mexico. This large input dominates the biological processes of the region. The “new” nitrogen from the river stimulates high levels of phytoplankton production which in turn support high rates of bacterial production, protozoan and metazoan grazing, and fisheries production. A portion of the particulate organic matter produced in the pelagic food web sinks out of the euphotic zone where it contributes to high rates of oxygen consumption in the bottom waters of the inner shelf, resulting in the development of an extensive zone of hypoxia each summer. In spite of the significance of this river system to the coastal ocean of the northern gulf, we do not have an adequate understanding of the inputs, processing and ultimate fates of river nitrogen. Here we review available literature on this important system and propose a conceptual model showing how biological processes evolve in the river plume between the point of discharge and the point where plume waters are fully diluted by mixing with oceanic water.     

Seasonal variation of riverine nutrient inputs in the northern Bay of Biscay (France), and patterns of marine phytoplankton response

Seasonal variations in nutrient inputs are described for the main rivers (Loire and Vilaine) flowing into the northern Bay of Biscay. The river plumes are high in N/P ratio in late winter and spring, but not in the inner plume during the summer. Conservative behavior results in most nutrients entering the estuary and eventually reaching the coastal zone. Temporal and spatial aspects of phytoplankton growth and nutrient uptake in the northern Bay of Biscay distinguish the central area of salinity 34 from the plume area. The first diatom bloom appears offshore in late winter, at the edge of the river plumes, taking advantage of haline stratification and anticyclonic “weather windows.” In spring, when the central area of the northern shelf is phosphorus-limited, small cells predominate in the phytoplankton community and compete with bacteria for both mineral and organic phosphorus. At that period, river plumes are less extensive than in winter, but local nutrient enrichment at the river mouth allows diatom growth. In summer, phytoplankton become nitrogen-limited in the river plumes; the central area of the shelf is occupied by small forms of phytoplankton, which are located on the thermocline and use predominantly regenerated nutrients.     

Nutrient and organic matter patterns across the Mackenzie River, estuary and shelf during the seasonal recession of sea-ice

Suspended material, nutrients and organic matter in Mackenzie River water were tracked along a 300 km transect from Inuvik (Northwest Territories, Canada), across the estuarine salinity gradient in Kugmallit Bay, to offshore marine stations on the adjacent Mackenzie Shelf. All particulates measured (SPM, POC, PN, PP) declined by 87–95% across the salinity gradient and levels were generally below conservative mixing. Organic carbon content of suspended material decreased from 3.1% in the river to 1.7% in shelf surface waters while particulate C:N concurrently decreased from 17.1 to 8.6. Nitrate and silicate concentrations declined by more than 90% across the salinity gradient, with nitrate concentrations often below the conservative mixing line. Phosphate concentrations increased from 0.03 μmol/L in the river to 0.27 μmol/L over shelf waters, thereby changing the inorganic nutrient regime downstream from P to N limitation. Dissolved organic carbon decreased conservatively offshore while dissolved organic N and P persisted at high levels in the Mackenzie plume relative to river water, increasing 2.7 and 25.3 times respectively. A deep chlorophyll-a maximum was observed at two offshore stations and showed increases in most nutrients, particulates and organic matter relative to the rest of the water column. During river passage through the Mackenzie estuary, particulate matter, dissolved organic carbon and inorganic nutrients showed sedimentation, dilution and biological uptake patterns common to other arctic and non-arctic estuaries. Alternatively, inorganic content of particles increased offshore and dissolved organic N and P increased substantially over the shelf, reaching concentrations among the highest reported for the Arctic Ocean. These observations are consistent with the presence of a remnant ice-constrained (‘stamukhi’) lake from the freshet period and a slow flushing river plume constrained by sea-ice in close proximity to shore. Nutrient limitation in surface shelf waters during the ARDEX cruise contributed to the striking deep chlorophyll-a maximum at 21 m where phytoplankton communities congregated at the margin of nutrient-rich deep ocean waters.     

Asymmetry of Columbia River tidal plume fronts

Columbia River tidal plume dynamics can be explained in terms of two asymmetries related to plume-front depth and internal wave generation. These asymmetries may be an important factor contributing to the observed greater primary productivity and phytoplankton standing crop on the Washington shelf. The tidal plume (the most recent ebb outflow from the estuary) is initially supercritical with respect to the frontal internal Froude number F_R on strong ebbs. It is separated from the rotating plume bulge by a front, whose properties are very different under upwelling vs. downwelling conditions. Under summer upwelling conditions, tidal plume fronts are sharp and narrow (< 20–50 m wide) on their upwind or northern side and mark a transition from supercritical to subcritical flow for up to 12 h after high water. Such sharp fronts are a source of turbulent mixing, despite the strong stratification. Because the tidal plume may overlie newly upwelled waters, these fronts can mix nutrients into the plume. Symmetry would suggest that there should be a sharp front south of the estuary mouth under summer downwelling conditions. Instead, the downwelling tidal plume front is usually diffuse on its upstream side. Mixing is weaker, and the water masses immediately below are low in nutrients. There is also an upwelling–downwelling asymmetry in internal wave generation. During upwelling and weak wind conditions, plume fronts often generate trains of non-linear internal waves as they transition from a supercritical to a subcritical state. Under downwelling conditions, internal wave release is less common and the waves are less energetic. Furthermore, regardless of wind conditions, solition formation almost always begins on the south side of the plume so that the front “unzips” from south to north. This distinction is important, because these internal waves contribute to vertical mixing in the plume bulge and transport low-salinity water across the tidal plume into the plume bulge.F_R and plume depth are key parameters in distinguishing the upwelling and downwelling situations, and these two asymmetries can be explained in terms of potential vorticity conservation. The divergence of the tidal outflow after it leaves the estuary embeds relative vorticity in the emerging tidal plume water mass. This vorticity controls the transition of the tidal plume front to a subcritical state and consequently the timing and location of internal wave generation by plume fronts.     

A conceptual model of the strongly tidal Columbia River plume

The Columbia River plume is typical of large-scale, high discharge, mid-latitude plumes. In the absence of strong upwelling winds, freshwater from the river executes a rightward turn and forms an anticyclonic bulge before moving north along the Washington coast. In addition to the above dynamics, however, the river plume outflow is subject to large tides, which modify the structure of the plume in the region near the river mouth. Observations based on data acquired during a summer 2005 cruise indicate that the plume consists of four distinct water masses; source water at the lift-off point, and the tidal, re-circulating and far-field plumes. In contrast to most plume models that describe the discharge of low-salinity estuary water into ambient high-salinity coastal water, we describe the Columbia plume as the superposition of these four plume types.We focus primarily on a conceptual summary of the dynamics and mutual interaction of the tidal and re-circulating plumes. The new tidal plume flows over top of the re-circulating plume and is typically bounded by strong fronts. Soon after the end of ebb tide, it covers roughly 50–100% of the re-circulating plume surface area. The fronts may penetrate well below the re-circulating plume water and eventually spawn internal waves that mix the re-circulating plume further. The re-circulating plume persists throughout the tidal cycle and corresponds to a freshwater volume equivalent to 3–4 days of river discharge. Finally, the plume water masses are distinguished from one another in term of surface chlorophyll concentration, suggesting that the above classification may also describe different biological growth regimes. The low-salinity re-circulating plume serves as an extension of the estuary into the coastal ocean, or an “estuary at sea”, because residence times during periods of high river flow are greater than those in the estuary.     

Daily and seasonal variations of the partial pressure of CO2 in surface seawater along Belgian and southern Dutch coastal areas

The variations of the partial pressure of CO₂ (pCO₂) and related parameters were determined in surface seawater along the Belgian coast, from January 1995 to June 1996, at both daily and seasonal time scales. The distribution of pCO₂ in this area is regulated by river input from the Scheldt, biological activity and hydrodynamics. The contribution of each of these processes varies as a function of the considered time scale: (i) the daily variation of pCO₂ depends on the tide although modulated by the biological diel cycle; (ii) the seasonal variation of pCO₂ depends on the input from the Scheldt and the seasonal variations of phytoplanktonic biomass. During winter, the plume of the river Scheldt is oversaturated in pCO₂ with respect to the atmosphere. During spring and summer, phytoplankton blooms occur both in the lower Scheldt estuary and in the river plume and may lead to undersaturation of pCO₂ in the easternmost area of the river plume. However, the degradation of phytoplankton induces oversaturation of pCO₂, in the westernmost area of the plume. Furthermore, the inter-annual variation of pCO₂ depends partly on the fluctuations of the discharge of the Scheldt. Our preliminary results strongly suggest that, on an annual basis, the Scheldt plume behaves as a net source of CO₂ to the atmosphere.     

Environmental changes in the western Black Sea related to anthropogenic and natural conditions

The Black Sea northwestern shelf (NWS) ecosystem has been subjected to the strongest anthropogenic pressure of the entire Black Sea as about 80% of the freshwater runoff is discharged there.This paper presents a review of the global environmental changes related mainly to increased eutrophication in the western Black Sea basin.A case study (CoMSBlack 92a cruise) attempts to highlight the interaction of some natural and anthropogenic factors responsible for specific chemical and biological features in the western Black Sea environment. The significance of processes located near the Danube river mouth, which, due to their relatively small space scale, have not been recognized before, is clarified. Hydrological processess of specific importance to the distribution and transformation of antropogenic inputs are river plume dynamics, coastal upwelling and mixing and downwelling over the shelf break and slope in this part of the basin. A layer of low hydrological variability (“conservative layer”) appears to be a natural feature of the area, hypothesized to precondition specific biological and chemical processes there.The results suggest that the interplay between the Danube anthropogenic nutrient load with the natural hydrological fronts and gradients provides opportunities for enhanced biological activity thus contributing to the global environmental changes in the Black Sea NWS.     

Influence of the Mackenzie River plume on the sinking export of particulate material on the shelf

We examined the influence of the Mackenzie River plume on sinking fluxes of particulate organic and inorganic material on the Mackenzie Shelf, Canadian Arctic. Short-term particle interceptor traps were deployed under the halocline at 3 stations across the shelf during fall 2002 and at 3 stations along the shelf edge during summer 2004. During the two sampling periods, the horizontal patterns in sinking fluxes of particulate organic carbon (POC) and chlorophyll a (chl a) paralleled those in chl a biomass within the plume. Highest sinking fluxes of particulate organic material occurred at stations strongly influenced by the river plume (maximum POC sinking fluxes at 25 m of 98 mg C m^− 2 d^− 1 and 197 mg C m^− 2 d^− 1 in 2002 and 2004, respectively). The biogeochemical composition of the sinking material varied seasonally with phytoplankton and fecal pellets contributing considerably to the sinking flux in summer, while amorphous detritus dominated in the fall. Also, the sinking phytoplankton assemblage showed a seasonal succession from a dominance of diatoms in summer to flagellates and dinoflagellates in the fall. The presence of the freshwater diatom Eunotia sp. in the sinking assemblage directly underneath the river plume indicates the contribution of a phytoplankton community carried by the plume to the sinking export of organic material. Yet, increasing chl a and BioSi sinking fluxes with depth indicated an export of phytoplankton from the water column below the river plume during summer and fall. Grazing activity, mostly by copepods, and to a lesser extent by appendicularians, appeared to occur in a well-defined stratum underneath the river plume, particularly during summer. These results show that the Mackenzie River influences the magnitude and composition of the sinking material on the shelf in summer and fall, but does not constitute the only source of material sinking to depth at stations influenced by the river plume.     

Patchiness in the Fly River plume in Torres Strait

Oceanographic studies were carried out from August 1994 to March 1995 on the intrusion of the Fly River plume in Torres Strait. Measurements at offshore coral reefs revealed an event of decreased salinity (≈24) while salinity of the water over the reefs fluctuated between 30–34 the rest of the time. Modelling suggests that this event resulted from the reversal of longshore currents advecting old river plume water back past the river mouth. There the new river water mixed with the old river plume water generating a patch of low-salinity water. While such events may be infrequent, they have the potential to leave a terrestrial signature on offshore coral reefs, in terms of (1) an input of terrigenous sediment and (2) the possible incorporation of riverine particulate metal into the food chain. The impact during an intrusion event may be significant. In the long term the riverine material is diluted in calcareous sediment produced throughout the year by bio-erosion of coral reefs.     

Dynamics of inorganic nutrient species in the Bohai seawaters

Within the frame of a Sino-German Joint Research Program, two cruises of “R/V Dong Fang Hong 2” were carried out in September–October 1998 and April–May 1999, respectively, to understand the dynamics of nutrients in the Bohai. Nutrient species (NO₃⁻, NO₂⁻, NH₄⁺, PO₄³⁻ and SiO₃²⁻) are determined colorimetrically on board for five anchor and 30 grid stations. In situ incubation experiments are performed to determine planktonic nutrient uptake and benthic exchange flux. Nutrient concentrations display short-term variability and seasonal change in the Bohai, with higher levels in shallow coastal waters than in the Central Bohai. The influence of riverine discharge on nutrient levels can be seen from salinity isopleths, nutrient distribution and species ratios. Near-bottom (nb) waters have similar nutrient concentrations as to the surface waters in the Central Bohai, whereas stratification takes place in the Bohai Strait and North Yellow Sea. In situ incubation experiments provide evidence that the uptake ratio (i.e. N, P) by phytoplankton is proportional to the ratios among nutrient species in ambient waters. Based on the data of this study and previously publications, a preliminary estimate of nutrient budgets via riverine input and atmospheric deposition is established. The results indicate that atmospheric deposition gains importance over rivers in delivering nutrients into the Bohai and sustain the new production, following recent decrease in riverine inflow caused by drought periods in North China and damming practices. A historical review of nutrient data indicates that concentrations of nitrogen increase and phosphorus and silica decrease in the Central Bohai over last 40 years. This potentially has an important influence on the health of ecosystem in Bohai (e.g. food web and community structure), though further study is needed to examine the scenario in more detail.     

Quasi-simultaneous observation of currents,salinity and nutrients in the Changjiang (Yangtze River) plume on the tidal timescale

During both the spring- and the neap-tide periods of November 2005, quasi-simultaneous observations were carried out by six boats over 26 h at 12 stations in the Changjiang (Yangtze River) plume. The simultaneous observations provided the actual distribution isopleths of salinity and nutrients that displayed considerable intra-tidal variations at surface, especially in the southeastern section of the study area. The lack of synopticity in sampling might lead to large discrepancies of the interpolated contours of salinity from the actual distribution isopleths. No clear flood-ebb asymmetry of salinity stratification was observed; whereas at inner stations, surface-to-bottom bulk velocity difference (current layering) always tended to be greater during the ebb fraction of a semidiurnal cycle. At a given station, the weaker neap tides commonly induced stronger salinity stratification, less intra-tidal variability of salinity and nutrients, and less intrusion of bottom saltwater. Nutrients (SiO₃^2?, NO₃^?, and PO₄^3?) showed more nonconservative behaviors during the neap tides, presumably as a result of the prolonged residence time of seawater and decreased suspended particulate matter levels than during the spring tides.     

Bacterial production and microbial food web structure in a large arctic river and the coastal Arctic Ocean

Globally significant quantities of organic carbon are stored in northern permafrost soils, but little is known about how this carbon is processed by microbial communities once it enters rivers and is transported to the coastal Arctic Ocean. As part of the Arctic River-Delta Experiment (ARDEX), we measured environmental and microbiological variables along a 300 km transect in the Mackenzie River and coastal Beaufort Sea, in July–August 2004. Surface bacterial concentrations averaged 6.7 × 10⁵ cells mL^− 1 with no significant differences between sampling zones. Picocyanobacteria were abundant in the river, and mostly observed as cell colonies. Their concentrations in the surface waters decreased across the salinity gradient, dropping from 51,000 (river) to 30 (sea) cells mL^− 1. There were accompanying shifts in protist community structure, from diatoms, cryptophytes, heterotrophic protists and chrysophytes in the river, to dinoflagellates, prymnesiophytes, chrysophytes, prasinophytes, diatoms and heterotrophic protists in the Beaufort Sea.Size-fractionated bacterial production, as measured by ³H–leucine uptake, varied from 76 to 416 ng C L^− 1 h^− 1. The contribution of particle-attached bacteria (> 3 µm fraction) to total bacterial production decreased from > 90% at the Mackenzie River stations to < 20% at an offshore marine site, and the relative importance of this particle-based fraction was inversely correlated with salinity and positively correlated with particulate organic carbon concentrations. Glucose enrichment experiments indicated that bacterial metabolism was carbon limited in the Mackenzie River but not in the coastal ocean. Prior exposure of water samples to full sunlight increased the biolability of dissolved organic carbon (DOC) in the Mackenzie River but decreased it in the Beaufort Sea.Estimated depth-integrated bacterial respiration rates in the Mackenzie River were higher than depth-integrated primary production rates, while at the marine stations bacterial respiration rates were near or below the integrated primary production rates. Consistent with these results, P_CO2 measurements showed surface water supersaturation in the river (mean of 146% of air equilibrium values) and subsaturation or near-saturation in the coastal sea. These results show a well-developed microbial food web in the Mackenzie River system that will likely convert tundra carbon to atmospheric CO₂ at increasing rates as the arctic climate continues to warm.     

Validation of the 3D biogeochemical model MIRO&CO with field nutrient and phytoplankton data and MERIS-derived surface chlorophyll a images

This paper presents results obtained with MIRO&CO-3D, a biogeochemical model dedicated to the study of eutrophication and applied to the Channel and Southern Bight of the North Sea (48.5°N–52.5°N). The model results from coupling of the COHERENS-3D hydrodynamic model and the biogeochemical model MIRO, which was previously calibrated in a multi-box implementation. MIRO&CO-3D is run to simulate the annual cycle of inorganic and organic carbon and nutrients (nitrogen, phosphorus and silica), phytoplankton (diatoms, nanoflagellates and Phaeocystis), bacteria and zooplankton (microzooplankton and copepods) with realistic forcing (meteorological conditions and river loads) for the period 1991–2003. Model validation is first shown by comparing time series of model concentrations of nutrients, chlorophyll a, diatom and Phaeocystis with in situ data from station 330 (51°26.00′N, 2°48.50′E) located in the centre of the Belgian coastal zone. This comparison shows the model's ability to represent the seasonal dynamics of nutrients and phytoplankton in Belgian waters. However the model fails to simulate correctly the dissolved silica cycle, especially during the beginning of spring, due to the late onset (in the model) of the early spring diatom bloom. As a general trend the chlorophyll a spring maximum is underestimated in simulations. A comparison between the seasonal average of surface winter nutrients and spring chlorophyll a concentrations simulated with in situ data for different stations is used to assess the accuracy of the simulated spatial distribution. At a seasonal scale, the spatial distribution of surface winter nutrients is in general well reproduced by the model with nevertheless a small overestimation for a few stations close to the Rhine/Meuse mouth and a tendency to underestimation in the coastal zone from Belgium to France. PO₄ was simulated best; silica was simulated with less success. Spring chlorophyll a concentration is in general underestimated by the model. The accuracy of the simulated phytoplankton spatial distribution is further evaluated by comparing simulated surface chlorophyll a with that derived from the satellite sensor MERIS for the year 2003. Reasonable agreement is found between simulated and satellite-derived regions of high chlorophyll a with nevertheless discrepancies close to the boundaries.     

Seasonal variability of dissolved major and trace elements in the Gaoping (Kaoping) River Estuary, Southwestern Taiwan

To gain a better understanding of the geochemical behavior of trace elements in estuary and to examine seasonal variations in associated chemical fluxes, more than 50 water samples were collected near the mouth of the Gaoping (formerly spelled Kaoping) River, a major river in southwestern Taiwan. These samples, collected in typical dry and wet tropical monsoon seasons during 1999–2004, were analyzed for dissolved major and trace elements and Sr isotopes. Our results show that dissolved Na, Mg, Ca and Cl behave conservatively along the salinity gradient and display significantly larger fluxes in the wet seasons. Vertical profiles of the major elements reveal mainly two end-member mixing between the riverine freshwater and the seawater. Trace elements of B, Sr and U also display conservative distribution in the vertical profiles. In contrast, dissolved Ba and Mn were affected by uptake/release processes involving groundwater, benthic flux and water/sediment interactions. ⁸⁷Sr/⁸⁶Sr ratios also support a scenario of mixing between a more radiogenic continental source and the seawater. It appears that the wet season samples have higher trace element concentrations due to inputs from topsoils and atmospheric dusts. This implies that chemical compositions in river waters respond sensitively to regional climatic changes. The observed high fluxes of B and Sr in the Gaoping (Kaoping) River emphasize the potential impact of mountainous rivers on the global oceanic mass balance of these constituents.     

Benthic assemblages of a temperate estuarine system in South America: Transition from a freshwater to an estuarine zone

The objectives of the present study were to describe the species composition, diversity and distribution of the zoobenthic assemblages, to estimate the abundance and biomass of the dominant species, and to identify the main environmental factors determining the distribution patterns of the invertebrates from a freshwater to an estuarine zone in a temperate estuary of South America. The Río de la Plata estuary is a microtidal system characterized by a high concentration of suspended solids. Fifty-three taxa of meso- and macro-invertebrates were identified in the samples collected during November and December 2001. Molluscs, annelids, crustaceans and nematodes were found at 90% of the sampling sites. Molluscs comprised up to about 90% of the total zoobenthos biomass: the remaining percentage corresponded mainly to annelids and less to nematodes and crustaceans. An ecocline along the salinity gradient could be observed for the benthic assemblages from the freshwater to the estuarine zone in Rio de la Plata. A Canonical Correspondence Analysis shows that results from sampling sites in the outer zone were strongly related to salinity, depth and pH and less to oxygen and percentage of clay. The results from stations in the inner zone, and part of the middle zone, were mainly related to the occurrence of sand and contents of NH₄⁺–N, NO₃⁻–N, and PO₄³⁻–P.     

Nitrogenous discharges to the eastern Gulf of Finland, the Baltic Sea: Elemental flows, stable isotope signatures, and their estuarine modification

We studied the nutrient input to the Gulf of Finland via River Neva, the largest river discharging freshwater to the Baltic Sea, and characterised the isotopic signatures (¹⁵N, ¹⁸O, ¹³C) in dissolved and particulate substances (NO₃⁻, PON, POC, DIC) in the River Neva over two seasonal cycles, as well as in samples from St. Petersburg wastewater treatment plants (NO₃⁻, NH₄⁺, PON, POC). These riverine and municipal discharges account for 40% of terrestrial inorganic N loading to the Gulf of Finland, representing annually 7% of the total nitrogen pool in the water mass of the whole Gulf. To describe and evaluate the modification of these isotopic signals along a Gulf of Finland transect towards the Baltic Proper, two cruises were arranged, one in late spring after the annual maximum in River Neva runoff, and one in autumn, in the late phase of the annual growth season.River Neva nitrate signatures of ¹⁵N and ¹⁸O indicated major agricultural fertilizer origin of nitrogen, and the isotopic composition was clearly lighter (δ¹⁵N-NO₃⁻ mean of 2.4‰ air) than previously measured from more southern rivers discharging into the Baltic Sea. Because of the light composition of the River Neva N source, close to the ¹⁵N signatures of the open Gulf, as well as of the efficient depletion of the inorganic load already in the innermost estuary, straightforward end-member tracer analysis of the transport of N in the basin is problematic. St. Petersburg wastewater ammonium showed, however, high δ¹⁵N values (ca. 13‰), which gives a first estimate of 5.8‰ for δ¹⁵N of the easternmost estuarine total inorganic N source. The available sediment data from the basin (δ¹⁵N 6 to 8‰) somewhat exceeds the average source signature. This emphasizes the significance of biological transformation processes, most importantly assimilation of inorganic nitrogen, food web interactions and denitrification, which all involve isotopic fractionation, for the mass balance models describing the dynamics of the sources and sinks of the N cycle of the basin.     

Environmental changes and the responses of the ecosystems of the Yellow Sea during 1976–2000

The changes in the environmental features of the Yellow Sea during the last 25 years of the 20th century were studied using a set of seasonally monitored data along a transect (at 36°N) maintained by the State Oceanic Administration of China. The data included the ocean temperature (T), salinity (S) and biogenic elements, such as dissolved oxygen (DO), phosphorus (PO₄-P), silicon (SiO₃-Si) and dissolved inorganic nitrogen (DIN).The seasonal (summer and winter) values and the annual mean of these elements showed significant changes during the monitored period. Time series of T, S, DIN and N:P ratios exhibited positive trends, while those of DO, P and Si exhibited negative trends. During this period, the annual mean of T and DIN in the Yellow Sea increased by 1.7 °C and 2.95 μmol L⁻¹, respectively, while those of DO, P and Si decreased by 59.1, 0.1 and 3.93 μmol L⁻¹, respectively. In the 1980s, particularly in between 1985 and 1989, concentrations of P and Si dropped to near the ecological threshold for growth of diatoms. The N:P ratio increased from 4 in 1984 to over 16 in 2000. The climate trend coefficients, R_xt, for these time series are all above 0.43 with significance levels of 95%, except for salinity. The increases in T were consistent with the recent climate warming in northern China and the adjacent seas, i.e. the Bohai Sea and the East China Sea. The reduction of DO was probably attributable to the increase in T and decrease in primary production in these regions. The positive trend of DIN was mainly attributable to precipitation and partly to Changjiang River discharge. The negative concentration trends of P and Si were due to the decreases in their concentrations in seawater that flowed to the Yellow Sea from the Bohai Sea. As a result, N:P ratios greatly increased in the seawater of the Yellow Sea.Moreover, some important responses of the Yellow Sea ecosystems to the changes in physical variables and chemical biogenic elements were obviously displayed. These responses include strengthening nutrient limitation, decreasing chlorophyll a, primary production and phytoplankton abundance, succession of dominant phytoplankton species from diatoms to non-diatoms, changes in fish community structure and species diversity.     

Inter-annual variability of hypoxic conditions in a shallow estuary

Water quality data from two monitoring programs in the Pamlico River Estuary (PRE) were analyzed for dissolved oxygen (DO), salinity, temperature, and nutrient concentrations. Data were collected bi-weekly at 8 stations from 1997 to 2003 by East Carolina University and continuously at three stations from 1999 to 2003 by the U.S. Geological Survey. Hypoxic conditions were observed mostly in the upper to middle estuary, but the frequency of hypoxic events varied between years. During June to October in 1997–1999 (referred to as the oxic summers) bottom water hypoxia (DO < 2 mg l^− 1) was found in 8.7% of the observations. By contrast, during June to October in 2001–2003 (referred to as the hypoxic summers), 37.9% of the total measurements had DO concentrations less than 2 mg l^− 1. The more frequent and/or prolonged hypoxic conditions during the hypoxic summers were closely associated with stronger salinity stratification and greater loadings of nutrient and particulate matter.Salinity stratification appeared to be governed by patterns of freshwater discharge, and frequency of wind mixing events. The “oxic” summers were characterized by continuous low freshwater inflow (except one extremely high flow event due to hurricanes), stronger northeastward wind, and more frequent wind mixing events. In contrast, the hypoxic summers were characterized by frequent moderate freshwater inflow events, and fewer wind mixing events.The greater loadings of nutrient (nitrate, ammonium, and phosphate) and particulate matter during the hypoxic summers were primarily due to higher river discharges. At the head of the PRE, no significant differences were found in concentrations of nutrient and particulate nitrogen between the oxic and the hypoxic summers. In addition, chlorophyll a concentrations were averaged above 30 μg l^− 1 (maximum 167 μg l^− 1) during the hypoxic summers, significantly higher than those during the oxic summers (averaged around 15 μg l^− 1).     

Turbulence in coastal fronts near the mouths of Block Island and Long Island Sounds

Measurements of turbulence were performed in four frontal locations near the mouths of Block Island Sound (BIS) and Long Island Sound (LIS). These measurements extend from the offshore front associated with BIS and Mid-Atlantic Bight Shelf water, to the onshore fronts near the Montauk Point (MK) headland, and the Connecticut River plume front. The latter feature is closely associated with the major fresh water input to LIS. Turbulent kinetic energy (TKE) dissipation rate, ε, was obtained using shear probes mounted on an autonomous underwater vehicle. Offshore, the BIS estuarine outflow front showed, during spring season and ebb tide, maximum TKE dissipation rate, ε, estimates of order 10^− 5 W/kg, with background values of order 10^− 6 to 10^− 9 W/kg. Edwards et al. [Edwards, C.A., Fake, T.A., and Bogden, P.S., 2004a. Spring–summer frontogenesis at the mouth of Block Island Sound: 1. A numerical investigation into tidal and buoyancy-forced motion. Journal of Geophysical Research 109 (C12021), doi:10.1029/2003JC002132.] model this front as the boundary of a tidally driven, baroclinically adjusted BIS flow around the MK headland eddy. At the entrance to BIS, near MK, two additional fronts are observed, one of which was over sand waves. For the headland site front east of MK, without sand waves, during ebb tide, ε estimates of 10^− 5 to 10^− 6 W/kg were observed. The model shows that this front is at the northern end of an anti-cyclonic headland eddy, and within a region of strong tidal mixing. For the headland site front further northeast over sand waves, maximum ε estimates were of order 10^− 4 W/kg within a background of order 10^− 7–10^− 6 W/kg. From the model, this front is at the northeastern edge of the anti-cyclonic headland eddy and within the tidal mixing zone. For the Connecticut River plume front, a surface trapped plume, during ebb tide, maximum ε estimates of 10^− 5 W/kg were obtained, within a background of 10^− 6 to 10^− 8 W/kg. Of all four fronts, the river plume front has the largest finescale mean-square shear, S² ~ 0.15 s^− 2. All of the frontal locations had local values of the buoyancy Reynolds number indicating strong isotropic turbulence at the dissipation scales. Local values of the Froude number indicated shear instability in all of the fronts.