REGULATION OF THE INTERFERON－INDUCIBLE PROTEIN KINASEPKR AND2＇5＇ OLIGOADENYLATE SYNTHETASE BY A CATALYTICALLY INACTIVE PKR M

ＲＥＧＵＬＡＴＩＯＮＯＦＴＨＥＩＮＴＥＲＦＥＲＯＮ－ＩＮＤＵＣＩＢＬＥＰＲＯＴＥＩＮＫＩＮＡＳＥＰＫＲＡＮＤ２＇５＇ＯＬＩＧＯＡＤＥＮＹＬＡＴＥＳＹＮＴＨＥＴＡＳＥＢＹＡＣＡＴＡＬＹＴＩＣＡＬＬＹＩＮＡＣＴＩＶＥＰＫＲ　ＭＵＴＡＮＴＴＨＲＯＵＧＨ　Ｃ...     

THE LA ANTIGEN INHIBITS THE ACTIVATION OF THE INTERFERON－INDUCIBLE PROTEIN KINASE PKR BY SEQUESTERING AND UNWINDING DOUBLE－ST

ＴＨＥＬＡＡＮＴＩＧＥＮＩＮＨＩＢＩＴＳＴＨＥＡＣＴＩＶＡＴＩＯＮＯＦＴＨＥＩＮＴＥＲＦＥＲＯＮ－ＩＮＤＵＣＩＢＬＥＰＲＯＴＥＩＮＫＩＮＡＳＥＰＫＲＢＹＳＥＱＵＥＳＴＥＲＩＮＧＡＮＤＵＮＷＩＮＤＩＮＧＤＯＵＢＬＥ－ＳＴＲＡＮＤＥＤＲＮＡＸｉａｏＱｉｕ...     

MULTIPLE ENHANCER ELEMENTS MEDIATE THE INDUCTION OF C-FOS BY ANGIOTENSIN Ⅱ

ＭＵＬＴＩＰＬＥＥＮＨＡＮＣＥＲＥＬＥＭＥＮＴＳＭＥＤＩＡＴＥＴＨＥＩＮＤＵＣＴＩＯＮＯＦＣ－ＦＯＳＢＹＡＮＧＩＯＴＥＮＳＩＮⅡＩＮＶＡＳＣＵＬＡＲＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＣｈｅｎＹａｎｑｕｎ；ＢａｒｔｅｋＲｙｄｚｅｗｓｋｉ，ＡｌｌｅｎＪ．...     

THE NEURONS DOUBLE-LABELLED BY SEROTONIN AND GLUTAMATE-IMMUNOREACTIVITY IN

ＴＨＥＮＥＵＲＯＮＳＤＯＵＢＬＥ－ＬＡＢＥＬＬＥＤＢＹＳＥＲＯＴＯＮＩＮＡＮＤＧＬＵＴＡＭＡＴＥ－ＩＭＭＵＮＯＲＥＡＣＴＩＶＩＴＹＩＮＭＥＤＵＬＬＡＲＹＲＡＰＨＥＮＵＣＬＥＩＰＲＯＪＥＣＴＩＮＧＴＯＴＨＥＣＥＲＥＢＥＬＬＡＲＣＯＲＴＥＸＯＦＴＨＥＫＩ...     

MULTIPLE TRANS-ACTING FACTORS MEDIATE THE INDUCTION OF C-FOS IN VASCULAR SMO

ＭＵＬＴＩＰＬＥＴＲＡＮＳ－ＡＣＴＩＮＧＦＡＣＴＯＲＳＭＥＤＩＡＴＥＴＨＥＩＮＤＵＣＴＩＯＮＯＦＣ－ＦＯＳＩＮＶＡＳＣＵＬＡＲＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＣｈｅｎＹａｎｑｕｎ；ＢａｒｔｅｋＲｙｄｚｅｗｓｋｉ，ＡｌｌｅｎＪ．Ｎａｆｔｉｌａｎ（Ｄｅ...     

COMPARATIVE STUDIES ON THE CORNEAL STRUCTURE OF MAMMAL ANIMALS：I．ULTRASTRUCTURE OF THE CORNEAL SUBSTANTIA PROPRIA IN GUINEA P

ＣＯＭＰＡＲＡＴＩＶＥ　ＳＴＵＤＩＥＳ　ＯＮ　ＴＨＥ　ＣＯＲＮＥＡＬ　ＳＴＲＵＣＴＵＲＥ　ＯＦ　ＭＡＭＭＡＬ　ＡＮＩＭＡＬＳ：Ｉ．ＵＬＴＲＡＳＴＲＵＣＴＵＲＥ　ＯＦ　ＴＨＥ　ＣＯＲＮＥＡＬ　ＳＵＢＳＴＡＮＴＩＡ　ＰＲＯＰＲＩＡ　ＩＮ　ＧＵＩＮＥＡ　Ｐ...     

REGULATED PHOSPHORYLATION OF THE GATA－2 DNA BINDINGPROTEIN IN ENDOTHELIAL CELLS

ＲＥＧＵＬＡＴＥＤＰＨＯＳＰＨＯＲＹＬＡＴＩＯＮＯＦＴＨＥＧＡＴＡ－２ＤＮＡＢＩＮＤＩＮＧＰＲＯＴＥＩＮＩＮＥＮＤＯＴＨＥＬＩＡＬＣＥＬＬＳＢｕＸｉｎＥ．Ｅ．Ｑｕｅｒｔｅｒｍｏｕｓ，Ｔ．Ｑｕｅｒｔｅｒｍｏｕｓ（ＤｅｐａｒｔｍｅｎｔｏｆＭｅｄｉｃｉｎｅ...     

PROTECTIVE EFFECT OF 3，6－DIMETHAMIDODIBENZOPYRIODONIUM CITRATE ON CALCIUM PARADOX IN THE ISOLATED GUINEA PIG HEART

ＰＲＯＴＥＣＴＩＶＥＥＦＦＥＣＴＯＦ３，６－ＤＩＭＥＴＨＡＭＩＤＯＤＩＢＥＮＺＯＰＹＲＩＯＤＯＮＩＵＭＣＩＴＲＡＴＥＯＮＣＡＬＣＩＵＭＰＡＲＡＤＯＸＩＮＴＨＥＩＳＯＬＡＴＥＤＧＵＩＮＥＡＰＩＧＨＥＡＲＴ（孙良起）（袁素芬）（马宏锐）（邱培伦）ＳｕｎＬ...     

IMPROVEMENT OF FLUORIMETRIC AND UV－VISIBLE SPECTROPHOTOMETRIC ASSAY METHODS FOR MEASURING NITRIC OXIDE AND／OR EDRF IN BLOOD

ＩＭＰＲＯＶＥＭＥＮＴＯＦＦＬＵＯＲＩＭＥＴＲＩＣＡＮＤＵＶ－ＶＩＳＩＢＬＥＳＰＥＣＴＲＯＰＨＯＴＯＭＥＴＲＩＣＡＳＳＡＹＭＥＴＨＯＤＳＦＯＲＭＥＡＳＵＲＩＮＧＮＩＴＲＩＣＯＸＩＤＥＡＮＤ／ＯＲＥＤＲＦＩＮＢＬＯＯＤＹａｎｇＦａｎ；ＬｉｕＰｎ;（Ｂｉ?..     

ALTERATIONS IN THE PROTEIN SYNTHESIS AND CONTENTS OF RIBOSOME AND POLYSOME IN LIVEROF SELENIUM－DEFICIENT RATS

ＡＬＴＥＲＡＴＩＯＮＳＩＮＴＨＥＰＲＯＴＥＩＮＳＹＮＴＨＥＳＩＳＡＮＤＣＯＮＴＥＮＴＳＯＦＲＩＢＯＳＯＭＥＡＮＤＰＯＬＹＳＯＭＥＩＮＬＩＶＥＲＯＦＳＥＬＥＮＩＵＭ－ＤＥＦＩＣＩＥＮＴＲＡＴＳ（毕红），（李同良）（贾锡安），（赵君庸）ＢｉＨｏｎｇ；Ｌｉ...     

THE EUKARYOTIC EXPRESSION OF HUMAN PERFORIN PROTEIN AND THE ESTABLISHMENT OF HYBRIDOMAS PRODUCING ANTI-HUMAN PERFORIN PROTEIN

Perforin is expressed mainly in activated cyto-toxic T lymphocytes (CTLs ) and natural killer(NK) cells. The human perforin gene has beencloned. The full length of its cDNA is l668bp, andthe mature HP protein is made up of 534 aminoacid residues with a molecular weight of 66Kd~75Kd. In CTLs and NK cells, perforin is stored incytoplasmic granules and is a major effector of cy-tolysis by these cells. Upon granules releasing,perforin monomers insert into the plasma mem-branes of target ce…     

硒对胎鼠肝细胞核酸及蛋白质合成的影响

本文报道了微量元素硒对培养纯种Wistar大白鼠胎肝细胞核酸及蛋白质合成的影响。观察了肝细胞培养基中不同硒水平对~3H-TdR(~3H-脱氧胸苷)、~(14)C-UR(~(14)C-尿苷)参入率的影响,同时还测定了培养基中白蛋白及甲胎蛋白(AFP)的含量。结果显示:细胞培养基中硒(Se)浓度在0.4～0.6μg/ml范围内使~3H-TdR、~(14)C-UR的参入率、白蛋白及甲胎蛋白含量均明显高于不补硒组。说明硒在这一浓度范围内能促进肝细胞DNA、RNA更新率及蛋白质合成。     

p53蛋白在慢性宫颈炎及宫颈癌中的表达

采用免疫组织化学ＳＰ法分别对５７例宫颈癌、８例慢性宫颈炎ｐ５３蛋白表达情况进行了检测,并以１１例正常宫颈鳞状上皮组织作对照。结果∶ｐ５３蛋白在宫颈癌、慢性宫颈炎中的表达率分别为７８．９％、３７．５％。正常对照均为阴性。ｐ５３蛋白的表达率在宫颈癌病理Ⅲ级中明显高于病理Ⅰ、Ⅱ级（Ｐ＜０．０５）;在溃疡型中的表达率明显低于菜花型、结节型（Ｐ＜０．０５）。提示∶ｐ５３蛋白在慢性宫颈炎中的表达与细胞增殖率升高有关;ｐ５３蛋白的表达率不仅与癌细胞的分化程度有关,而且与宫颈癌的大体分型有关。     

THE EXPERIMENTAL STUDY ON THE CELL APOPTOSIS AND EXPRESSION OF BCL-2 PROTEIN IN INTRACEREBRAL HEMORRHAGE IN MODEL OF RATS

Intra-cerebral hemorrhage is a common clinicaldisease,with a high mortality and morbidity.So itis one of the clinical hot topics.It has been foundinrecent years that there is a close relationship bet weenthe cell apoptosis and the course or prognosis of in-tra-cerebral hemorrhage.Bcl-2,as the apoptosis-adjusted gene,plays ani mportant role in the courseof cell apoptosis,but the mechanis min the cell ap-optosis in intra-cerebral hemorrhage remains un-clear.In this experi ment,with the model bui…     

涎腺粘液表皮样癌中角蛋白、波形蛋白和S-100蛋白的定位研究

用免疫组化法对37例涎腺粘液表皮样癌中角蛋白、波形蛋白和S-100蛋白定位进行观察,发现表皮样细胞和中间细胞呈角蛋白、波形蛋白和S-100蛋白阳性,粘液细胞呈角蛋白和S-100蛋白阳性。肿瘤团块中有些散在梭形、星形或不规则形细胞呈S—100蛋白强阳性;囊腔和腺管腔面细胞基部也有梭形、扁平或不规则形细胞呈S-100蛋白强阳性及波形蛋白和角蛋白阳性。在肿瘤细胞索外周还发现些梭形或扁平细胞是S-100蛋白、波形蛋白和角蛋白阳性。提示,涎腺粘液表皮样癌为上皮源性肿瘤,实质内存在肌上皮细胞,肌上皮细胞与中间细胞似有直接关联。     

bcl-2基因蛋白在外阴营养不良及外阴癌的表达

利用细胞凋亡抑制基因ｂｃｌ－２单克隆抗体及免疫组化ＬＳＡＢ法,对正常外阴皮肤、慢性外阴营养不良、不典型增生和外阴癌组织进行标记,发现ｂｃｌ－２蛋白在正常外阴皮肤基底层中有弱阳性表达;在增生型营养不良、不典型增生及外阴癌组织中表达率有不同程度增高,与外阴硬化苔癣型和混合型营养不良有显著差异（Ｐ＜０．０５,Ｐ＜０．０５,Ｐ＜０．０１）;而增生型与不典型增生ｂｃｌ－２基因蛋白表达无明显差异（Ｐ＞０．０５）;外阴癌的表达率最高;该基因蛋白表达与外阴癌细胞分化程度有关,与临床分期无关。