诊断超声对人早孕绒毛细胞DNA影响的定量研究

目的　探讨诊断超声经腹照射子宫内孕囊对人早孕绒毛细胞DNA的影响。方法　 60名拟行人流的早孕妇女随机分为照射 1 0min组和未照射组 ,各 3 0例。取绒毛组织后 ,以紫外分光光度法和3 2 P标记Alu探针打点杂交法定量检测绒毛细胞DNA单、双链断裂。结果　两种方法检测结果显示 ,照射组与对照组绒毛细胞单、双链DNA的量均无显著性差异 (P >0 .0 5 )。结论　诊断超声宫内照射孕囊 1 0min ,未引起人绒毛细胞DNA单、双链断裂 ,提示未引起绒毛细胞DNA损伤     

老年原发性骨质疏松患者血清IGF-1、IGF-BP-3水平与骨密度及骨折的关系

目的　探讨老年原发性骨质疏松患者血清胰岛素样生长因子1(IGF 1)和胰岛素样生长因子结合蛋白3 (IGF BP 3)水平与骨密度、骨折发生率的相关性。方法　选择老年原发性骨质疏松患者100 例,对照组60 例,进行血清IGF 1和IGF BP 3检测及髋部、腰椎骨密度测定,同时两组均进行生活方式及既往骨折史调查。结果　①骨质疏松组较对照组血清IGF 1水平显著降低,两组有显著性差异(P<0.05);②骨质疏松合并骨折患者,血清IGF 1 水平与对照组相比下降更为明显;③低钙摄入(<500 mg/d)、非负重户外运动时间减少和长期吸烟可使骨折发生的相对危险性增高;④血清甲状旁腺素(PTH)与髋部骨密度值呈负相关,血清IGF 1下降幅度与髋部骨密度值呈正相关,血清IGF BP 3影响不大。结论　不良生活方式及血清IGF 1降低,可促进老年骨质疏松的发生,并在一定程度上增加骨质疏松相关骨折的发生。     

电刺激活化牙槽骨成骨细胞增殖功能的实验研究

目的　观察电刺激对活化牙槽骨成骨细胞增殖功能的作用 ,探讨电刺激促进牙槽骨缺损修复的机理。方法　实验家兔分A、B两组 ,在双侧下颌牙槽突制作骨缺损区。A组用自制的位于右侧自凝塑胶牙托表面电极刺激实验家兔下颌右侧的骨缺损区 ;左侧及B组不做电刺激作为对照。结果　光镜下实验侧 1～ 2周即可见到大量增生的成骨细胞、成纤维细胞和丰富的毛细血管 ,其中成骨细胞呈多形性改变。 3～ 5周即有大量的骨小梁形成。而对侧和对照组骨小梁较少 ,且钙化差。电镜下实验侧可见到大量增生的成骨细胞胞浆内粗面内质网膨大 ,有大量的核蛋白颗粒 ,线粒体和高尔基体发达 ,呈现高电子密度电子云。结论　电刺激可促进成骨细胞的增殖分化而达到骨修复的目的     

EFFECT OF QUERCETIN ON CULTURED HUMAN VASCULARENDOTHELIAL CELLS

Quercetinisakindofflavonoidcompound.ItisdemonstratedthatQuelowersbloodpressure,b1oodlipidandcapillarypermeabilityanddilatescoronaryartery"'-Ouealsopossessesantioxida-tion,antineoplasticactivity,antiplatelet,aggrega-tioninducedbyadenosinediphosphate(ADP),thrombin,arachidonicacid,andtheinhibitionofproductionandreleaseofthromboxaneB2and5-hydroxytryptaminebyplatelet">-ItraisescAMPlevel,inhibitscGMP-dependentphosphodiesterasetoelevatecGMPlevelincellsc4i.Especially,thereisagreatprogressinanti…     

姜黄素对食管癌细胞系放射敏感性的影响及其机理

目的　探讨姜黄素对食管癌细胞系Eca10 9的放射增敏作用及其机制。方法　利用细胞克隆技术进行剂量 -存活曲线分析 ,末端脱氧核苷酰转移酶法检测细胞凋亡 ,并对细胞周期进行分析。结果　姜黄素组D0 值为 91.35cGy ,空白对照和空白药物组D0 值分别为 130 .0 7cGy和 138.37cGy ,(P <0 .0 5 )。流式细胞仪分析空白组和姜黄素组凋亡区阳性细胞分别为 1.0 1%和 87.5 8% (P <0 .0 5 ) ;经姜黄素处理后 ,与对照组相比 ,G0 1 期细胞比例降低 ,G2 + M期细胞比例增高。结论　姜黄素可以明显提高Eca 10 9细胞的放射敏感性 ,机制可能与其引起瘤细胞周期阻滞 ,诱导肿瘤细胞凋亡有关     

Identification of differentially expressed genes in two new human bladder carcinoma cell lines

Objective To screen and identify differentially expressed genes in two new human urothelial carcinoma cell lines, BLS-211 and BLX. Methods Suppression subtractive hybridization （SSH） was used to createa subtracted library, and clones were sequenced. Results Totally 13 over-expressed genes in BLX and 9 in BLS-211 cells were obtained, respectively. Among them, 18 were known genes and 4 were new ESTs （Expressed Sequence Tag）, and were collected by GenBank dbEST database （The access number was EB390424-7）. Conclusion SSH is a powerful method for the identification of differentially expressed genes. The differential expression of some BCG-associated genes in different ceils may be related to the different responses to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.     

不同表型的人宫颈癌细胞亚克隆株的基因表达谱

目的　采用基因芯片技术分析同一人宫颈癌细胞株来源 ,锚着依赖性不同的两个亚克隆细胞株的侵袭相关基因表达。方法　采用有限稀释法建立CS12 13细胞的CS0 3和CS0 7亚克隆细胞株 ,检测其在软琼脂中的集落形成率 ,分别用流式细胞术和免疫组化法观察细胞黏附分子CD4 4和E cadherin、Fibronetin的表达 ,并将CS0 3和CS0 7细胞的总RNA经逆转录获得的cDNA作为探针 ,与含 10 11个与细胞信号转导和细胞膜受体相关基因表达谱芯片杂交 ,信号用ScanArray 30 0 0扫描分析。结果　CS0 3和CS0 7细胞的集落形成率分别是 0和 38.5 % ,CD4 4表达分别是 35 .1%和 13.7% ;两者的基因表达谱差异明显 ,CS0 3细胞中表达上调的基因为 12个 ,CS0 7细胞中有 2 1个基因表达增加 ,其中一些与细胞增生、迁移、凋亡 ,细胞间信号传导和肿瘤转移相关 ,包括p34cdc2 、转化生长因子 β刺激蛋白 (TSC2 2 )、纤维蛋白酶原活性因子抑制剂 (PAI 1)和桥粒相关蛋白 (PININ)等。结论　同一来源的人宫颈癌细胞株中有多个差异表达基因 ,它们参与人宫颈癌细胞的表型特征 ,可能影响肿瘤的进展。     

THE CONSTRUCTION AND EXPRESSION OF THE MURINE SCFV GENE IN E. COLI AGAINST HUMAN CERVICAL CANCER

Objective To obtain the gene of murine Single chain Fv fragment （ScFv） against haman cervical cancer and to express it in E. coli. Methods The variable region gene fragments of the heavy and light chains, which were amplified respectively using recombinant DNA techniques from CsA125 hybridama cells, were spliced together through a flexible linker to ScFv against human cervical cancer. The ScFv genes were then cloned into expression vector pCANTAB 5E and expressed in E. coli HB2151 and TG1 respectively. The soluble ScFv were characterized by SDSPAGE and Western blot. The antigen-binding activities of the soluble and phage displayed ScFv were assayed by ELISA and cell immunohistochemical analysis. Results The expressed ScFv antibodies were soluble and phage displayed. soluble ScFv secreted and expressed in E. coli HB2151 induced by IPTG were confirmed with SDS-PAGE, Western blot and ELISA. The specific binding capacity of the soluble and phage displayed ScFv to the surface associated antigen of human cervical cancer cell line was further confirmed with immunohistochemical studies. Conclusion The soluble and phage displayed ScFv expressed in E. coll against haman cervical cancer showed high, specific affinity for the cervical cancer cell line surface associated antigen.     

单纯疱疹病毒胸苷激酶基因表达对人肝癌细胞的生长抑制

使用转导有单纯疱疹Ⅰ型病毒胸苷激酶（ＨＳＶ１－ＴＫ）基因的人肝癌ＳＭＭＣ－７７２１细胞株建立了裸鼠移植瘤模型，并进行了在体和体外试验性无环鸟苷（ＡＣＶ）治疗。结果发现基因转导后的肝癌细胞表现出对ＡＣＶ的敏感性，肝癌细胞生长抑制与ＡＣＶ的剂量有关；用药组裸鼠除平均瘤重明显低于对照组外，还可见细胞核分裂指数降低，伴有细胞变性和坏死。结果表明：ＨＳＶ１－ＴＫ基因经逆转录病毒转导后，用ＡＣＶ诱导表达明显抑制了人肝癌细胞的生长。     

逆转录病毒转移的β－半乳糖苷酶基因在成纤维细胞中的表达

为了研究逆转录病毒载体转移外源基因的实用性，我们采用小鼠白血病病毒（ＭｏＭＬＶ）载体将大肠杆菌的β－半乳糖苷酶基因（ｌａｃＺ）和抗新霉素（ＮｅｏＲ）基因转移到大鼠成纤维细胞内，对整合后的前病毒结构和基因表达进行了酶活性测定和ＤＮＡ分析。结果表明：ｌａｃＺ基因可以在大鼠皮肤成纤维细胞和２０８Ｆ细胞株中较长时间地表达；前病毒整合位点、靶细胞特性和整合后的载体结构与转入基因表达的稳定性紧密相关。