Association between the -1562 C/T polymorphism in the MMP-9 promoter and phenotype of esophageal squamous cell carcinoma in northern Chinese population

Objective To conduct a case-control study on the association of the nucleotide polymorphisms in the promoter region of the matrix metalloproteinase-9 (MMP-9) gene with phenotype of esophageal cancer. Methods All subjects were unrelated residents in northern regions of China. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the MMP-9 genotypes. Results The overall distribution of genotypes in the patients was not different from that in the controls (OR=0.77, 95% CI=0.45-1.34; P=0.36). There were no significant differences between the patients and the control subjects in terms of the distributions of sex and age, smoking status, alcohol dependence, pickled diet status, or history of environmental exposure. The patients were further examined with stratifications by age, sex, grade, depth of tumor invasion, lymphatic invasion, venous invasion and TNM staging. The results showed no pronounced association among the stratifications. Conclusion There is no significant association between the MMP-9 single nucleotide polymorphism genotypes and phenotype of esophageal cancer.     

肿瘤坏死因子α、白介素1β和舒马曲坦对大鼠三叉神经节离体培养后降钙素基因相关肽mRNA表达水平的影响

目的探讨肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和抗偏头痛药物舒马曲坦(Sumatriptan)对大鼠三叉神经节(TG)离体培养后降钙素基因相关肽(CGRP)mRNA表达水平的影响及其细胞内信号转导机制。方法将不同浓度的TNF-α、IL-1β、IL-2和Sumatriptan分别加入DMEM培养液中,与SD大鼠TG离体孵育24h;进一步将细胞内丝裂原激活的蛋白激酶(MAPK)信号转导系统ERK1/2、JNK、P38三条信号通路关键激酶特异性阻断剂PD98059、SP600125、SB239063加入DMEM培养液中,30min后分别加入有效浓度的炎性因子TNF-α和IL-1β后,与大鼠TG共同孵育24h,实时定量PCR(RT-PCR)法检测TG内CGRP-mRNA表达水平变化。结果 50μg/L的TNF-α、25μg/L的IL-1β可明显上调离体TG内CGRP-mRNA表达水平(P<0.05),0.1g/L和0.5g/L Sumatriptan能显著下调TG内CGRP-mRNA表达水平,而20～80μg/L的IL-2对CGRP-mRNA表达无影响。特异性阻滞剂SP600125、SB239063分别与50μg/L TNF-α、25μg/L IL-1β孵育24h后,CGRP-mRNA表达量较对照组显著降低(P<0.05)。结论 TG离体培养时,炎性因子TNF-α、IL-1β可显著上调CGRP-mRNA表达水平;一定浓度的舒马曲坦能下调CGRP-mRNA表达;JNK和P38信号转导通路参与了TNF-α、IL-1β诱导的TG内CGRP-mRNA上调过程。     

中国路面工程学术研究综述·2024

近年来,中国公路建设成果斐然,路网规模快速增长、关键技术不断突破。为进一步提升中国路面工程学科水平及国际影响力,推动路面工程可持续、高质量发展,从公路韧性评估与恢复、长寿命路面结构与材料、公路交通能源自洽、低环境影响公路技术、路面材料基因与高通量、公路数字化及智能化、公路智能检测与高性能养护等七大研究方向,系统梳理了近年来国内外路面工程的发展现状、前沿热点问题与未来发展趋势。具体围绕公路绿色化、韧性化、智能化、长寿命与交能结合等领域,遴选了20个热点研究方向,涵盖公路致灾因素及失效机理、公路韧性评估与恢复、公路韧性提升关键技术、公路长寿命路面结构足尺试验、公路结构与功能寿命增强技术、公路清洁能源采集技术、公路能源自洽设计、公路路域环境影响测试与评估方法、低环境影响路面新材料、沥青路面温拌再生技术、路面材料基因研究、路面材料跨尺度计算、路面材料基因与高通量研究、公路数字化建模技术、公路数字孪生仿真技术、公路运维数据驱动技术、公路探地雷达检测技术、路面性能轻量化检测、公路沥青路面抗滑性能检测及恢复技术、公路高性能预防养护技术等主要内容。该综述可为中国路面工程学科发展提供指引和参考,为路面工...     

英菲尼迪Q50 Eau Rouge

源自日产高端品牌英菲尼迪入门级轿车Q50的Eau Rouge拥有GT-R那台野兽般的双涡轮增压发动机经过重新优化最大功率可达41 8kW,0~100km/h加速不足4s……英菲尼迪Q50 Eau Rouge毋庸置疑地继承了众多"优良基因",动感的车身线条,强壮的造型和简洁的外观,无不诠释着英菲尼迪的独特审美。采用碳纤维材料制造的"前唇"虽然造型略嫌夸张,但好在很诱人。多辐式轮圈战斗风格浓重。     

hGPx-1-198Leu真核表达载体的构建与鉴定及在H9C2细胞中的表达

目的构建含有人GPx-1基因Pro198Leu多态的真核表达载体,为深入研究GPx-1基因Pro198Leu多态在相关疾病中的作用与机制提供依据。方法采用全基因合成的方法合成含多态位点的人GPx-1基因cDNA片段,同时在基因5′端和3′端分别引入BamHⅠ和NotⅠ酶切位点,通过限制性内切酶酶切目的片段和真核表达载体pEGFPN3,再用T4DNA连接酶将含有目的基因的片段定向插入到载体pEGFP-N3真核人巨细胞病毒启动子下游。连接产物转化至DH5α中。挑取克隆、提取质粒进行鉴定。将hGPx-1-198Leu真核表达载体转染HEK293细胞,观察转染效率,并采用RT-PCR检测转染重组载体后细胞中GPx-1的mRNA表达水平。重组载体转染H9C2细胞,观察补硒之后的GPx-1表达情况。结果获得目的基因cDNA片段全长869bp,PCR和测序鉴定载体中含有人GPx-1-198Leu基因cDNA。重组载体转染HEK293细胞后检测到GPx-1的mRNA水平比未转染组及空质粒转染组明显升高,为深入研究GPx-1基因Pro198Leu多态在相关疾病中的作用与机制奠定了基础。H9C2细胞在转染后GPx-1蛋白水平升高并且随着硒浓度的升高而升高。结论成功构建了含Pro198Leu多态位点的hGPx-1真核表达载体,并且也插入了保证该基因有效表达的硒代半胱氨酸插入序列。     

基于Double—Bagging决策树的基因微阵列数据研究

Bagging通过组合不稳定的分类器在很大程度上降低了"弱"学习算法的分类误差.基于Torsten等人提出的Double-Bagging算法.本文对其加以修改并应用于基因微阵列数据的处理.在给定的训练数据集和测试集上试验并比较了多种分类器,结果表明Double-Bassing决策树分类精确度优于Bagging决策树和C4.5算法.     

急性髓细胞白血病FLT3-ITD基因突变的检测及其临床意义

目的 探讨急性髓细胞白血病(acute myeloid leukemia,AML)患者人Fms样酪氨酸激酶3内部串联重复(Fms-like tyrosine kinase3-intenal tandem duplication,FLT3-ITD)基因突变及其临床意义.方法 采用聚合酶链反应-变性高效液相色谱(polymerase chain reaction-denaturing high performance liquid chromatography,PCR-DHPLC)方法检测60例初治AML患者和10例对照组骨髓FLT3-ITD基因突变.结果 60例AML患者FLT3-ITD突变阳性率21.67%(13/60),10例对照组均未检测到该突变;FAB各亚型FLT3-ITD突变率不同,有M3型突变率较高的趋势;FLT3-ITD阳性组外周血白细胞数、骨髓白血病细胞比例高于FLT3-ITD阴性组(P<0.01);FLT3-ITD阳性组完全缓解(CR)率低于FLT3-ITD阴性组(P<0.05).结论 FLT3-ITD基因突变多见于M3患者,FLT3-ITD阳性者具有外周血白细胞数、骨髓白血病细胞比例高、CR率低的特点.FLT3-ITD基因突变可作为预测AML预后的一个指标.     

IL-1β、幽门螺杆菌cagA与人慢性胃炎的关系

目的 探讨IL-1β、幽门螺杆菌cagA基因(cytotoxin A)和人慢性胃炎之间的相关性.方法 收集西安交通大学医学院第一附属医院胃镜检查的胃活检标本,经甲醛固定,制备石蜡切片,进行病理分型和IL-1β SABC免疫组化染色;同时作幽门螺杆菌培养,16s rRNA PCR鉴定,检测cagA基因.结果 慢性萎缩性胃炎组IL-1β表达的阳性率高于慢性浅表性胃炎组(P<0.05);慢性萎缩性胃炎组中,异型增生组IL-1β表达的阳性率高于肠腺化生组(P<0.05).幽门螺杆菌cagA基因阳性率在慢性萎缩性胃炎组和慢性浅表性胃炎组无显著性差异(P>0.05),异型增生组cagA基因阳性率和肠腺化生组亦无显著性差异(P>0.05).结论 IL-1β在慢性胃炎的转归和发展中具有比cagA基因更高的预测价值.     

Cloning and sequencing of a DNA fragment encoding N37 apoptotic peptide derived from p53

Objective It was reported that p53 apoptotic peptide (N37) could inhibit p73 gene through being bound with iASPP, which could induce tumor cell apoptosis. To further explore the function of N37, we constructed the cloning plasmid of DNA fragment encoding p53 (N37) apoptotic peptide by using DNA synthesis and molecular biology methods. Methods According to human p53 sequence from the GenBank database, the primer of p53(N37) gene was designed using Primer V7.0 software. The DNA fragment encoding p53 (N37) apoptotic peptide was amplified by using self-complementation polymerase chain reaction (PCR) method and cloned into the pGEM-T Easy vector. The constructed plasmid was confirmed by endonuclease analysis and sequencing. Results The insertion of objective DNA fragment was confirmed by plasmid DNA enzyme spectrum analysis, p53 (N37) gene was successfully synthesized chemically in vitro. The sequencing result of positive clone was completely identical to the human p53(N37) sequence in GenBank using BLAST software (http://www. ncbi. him. nih. gov/cgi-bin /BLASTn). Conclusion The cloning of DNA fragment encoding p53(N37) apoptotic peptide was constructed by using DNA synthesis and pGEM-T Easy cloning methods. With the constructed plasmid, we could further investigate the function of N37 peptide.     

表皮生长因子受体Ⅲ型变异体（EGFRvⅢ）多克隆抗体的制备及特异性

为了制备针对EGFRvⅢ的特异性多克隆抗体,在实验中利用耦联了血蓝蛋白的EGFRvⅢ特异性的PEP3合成短肽进行动物免疫,制备抗血清;构建了pET30a／E33XI原核表达载体,表达和纯化了融合蛋白His-E33（EGFRvⅢ的部分胞外区）;以此融合蛋白作为抗原制备了抗体纯化柱,通过柱上纯化的方法从抗血清中提取针对EGFRvⅢ的特异性抗体．利用U87、U251、HeLa和HEK-293细胞进行免疫组化实验来检验抗体的特异性．